成果/Result
- Highly Efficient Production of Soluble Proteins from Insoluble Inclusion Bodies by a Two-Step-Denaturing and Refolding Method被引量：70收藏
- 作者：Yang, Zhong Zhang, Linlin Zhang, Yan Zhang, Ting Feng, Yanye Lu, Xiuxiu Lan, Wenxian Wang, Jufang Wu, Houming Cao, Chunyang Wang, Xiaoning
- 机构：Fudan Univ;Chinese Acad Sci;E China Univ Sci & Technol;S China Univ Sci & Technol
- 来源：PLOS ONE 2011
- Mutant library construction in directed molecular evolution被引量：40收藏
- 作者：Wang, Tian-Wen Zhu, Hu Ma, Xing-Yuan Zhang, Ting Ma, Yu-Shu Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2006
- 关键词：directed molecular evolution error-prone PCR oligonucleotide-based mutagenesis DNA shuffling mutator strain somatic hypermutation
- Modeling of protein refolding from inclusion bodies被引量：15收藏
- 作者：Zhang, Ting Xu, Xiaojing Shen, Liang Feng, Yanye Yang, Zhong Shen, Yaling Wang, Jufang Jin, Weirong Wang, Xiaoning
- 机构：Natl Engn Ctr Biochip Shanghai;E China Univ Sci & Technol;Shandong Econ Univ;S China Univ Technol
- 来源：ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2009
- 关键词：Escherichia coli inclusion body statistical modeling protein refolding
- Genome-wide nonessential gene identification of Autographa californica multiple nucleopolyhedrovirus被引量：6收藏
- 作者：Yu, Yue Zhang, Tong Lu, Dongbo Wang, Jing Xu, Zhenhe Zhang, Yuanxing Liu, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：GENE 2023
- 关键词：Autographa californica multiple nucleopolyhedrovirus (AcMNPV) Baculovirus Gene deletion Insect cell Minimal genome
- An Automatic Refolding Apparatus for Preparative-Scale Protein Production被引量：5收藏
- 作者：Feng, Yanye Zhang, Ming Zhang, Linlin Zhang, Ting Ding, Jianfeng Zhuang, Yingping Wang, Xiaoning Yang, Zhong
- 机构：E China Univ Sci & Technol;Fudan Univ;Novoprotein Sci Inc
- 来源：PLOS ONE 2012
- Cooperation of FGF/MEK/ERK and Wnt/β-catenin pathway regulators to promote the proliferation and pluripotency of mouse embryonic stem cells in serum- and feeder-free conditions被引量：5收藏
- 作者：Zhang, Tong Chen, Huanyun Zhou, Yiran Dong, Wanghong Cai, Haibo Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2019
- 关键词：mESCs FGF/MEK/ERK pathway Wnt/beta-catenin pathway Small molecule regulator In vitro proliferation
- A lef5-deficient baculovirus expression system with no virion contamination and promoting secretion被引量：3收藏
- 作者：Hu, Die Xie, Xueman Zhang, Tong Yu, Yue Xu, Zhenhe Zhang, Yuanxing Liu, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2023
- 关键词：Autographa californica multiple nucleopolyhedrovirus Baculovirus expression vector Insect cell Lef5 Virus particle
- φC31-Mediated cassette exchange in Sf9 insect cells for stable expression被引量：1收藏
- 作者：Hu, Die Qian, Jingwen Zhang, Tong Yu, Yue Xu, Zhenhe Zhang, Yuanxing Liu, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2023
- 关键词：cassette exchange FACS insect cell line Sf9 phi C31
- YBac: A novel and smple baculovirus system for heterologous protein production in Spodoptera frugiperda 9 (Sf9) cells被引量：1收藏
- 作者：Yu, Yue Yang, Cancan Zhang, Tong Xu, Zhenhe Zhang, Yuanxing Liu, Qin
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2023
- 关键词：baculovirus gene deletion heterologous protein expression homologous recombination insect cells
