文献类型：期刊文献

英文题名：Construction of a whole-cell catalyst displaying a fungal lipase for effective treatment of oily wastewaters

作者：Song, Huiting[1,3];Zhou, Lichao[1];Zhang, Lujia[2];Gao, Bei[2];Wei, Dongzhi[2];Shen, Yaling[2];Wang, Rui[3];Madzak, Catherine[4];Jiang, Zhengbing[1,2]

机构：[1]Hubei Univ, Coll Life Sci, Hubei Key Lab Ind Biotechnol, Wuhan 430062, Peoples R China;[2]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Hubei Univ, Coll Resources & Environm Sci, Wuhan 430062, Peoples R China;[4]AgroParistech, CBAI, INRA, Micalis UMR1319, F-78850 Thiverval Grignon, France

年份：2011

卷号：71

期号：3-4

起止页码：166

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：;EI(收录号：20112314043029);WOS:【SCI-EXPANDED(收录号:WOS:000292228100013)】；

基金：This work was supported by the following grants: National Natural Science Foundation of China (50808069; 21076067), Program for New Century Excellent Talents in University (NCET), China Spark Program (2010GA760017), National Basic Research Program of China Program (2009CB724703), Research Project from Hubei Provincial Department of Education (Q20091010) and Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Activated sludge; Bioremediation; Cell surface display; Lipase; Oily wastewater; Yarrowia lipolytica

摘要：To combine the advantage of the oleaginous yeast Yarrowia lipolytica with the high activity of some fungal lipases for oily wastewater treatment, an effective lipase-displaying arming yeast was constructed using the flocculation functional domain of Saccharomyces cerevisiae as the protein anchor. To estimate the effect of the whole-cell oily wastewater treatment, the lipase-displaying arming yeast was added into an open activated sludge bioreactor. Within 72 h of whole-cell treatment, 96.9% of oil and 97.6% of chemical oxygen demand (COD) were removed, while only 87.1% of oil and 91.8% of COD were removed in control A (Y. lipolytica Polg was added), 45.1% of oil and 67.5% of COD were removed in control B (no cell was added) in 72 h. The lipase-displaying arming yeast exhibited remarkable oil removal and COD degradation effect compared with the control samples, exemplifying its application potential. (C) 2011 Elsevier B.V. All rights reserved.