文献类型：期刊文献

英文题名：Development of an auto-inducible expression system by nitrogen sources switching based on the nitrogen catabolite repression regulation

作者：Yan, Qin[1];Han, Laichuang[1];Liu, Xinyue[1];You, Cuiping[1];Zhou, Shengmin[2];Zhou, Zhemin[1]

机构：[1]Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2022

卷号：21

期号：1

外文期刊名：MICROBIAL CELL FACTORIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000788585700001)】；

基金：This work is supported by the International S&T Innovation Cooperation Key Project (2017YFE0129600), National Natural Science Foundation of China (32101014, 21878125), the Natural Sciences Foundation of Jiangsu (BK20200624, BK20210470), China Postdoctoral Science Foundation (2021M701461), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the 111 Project (No. 111-2-06), the Jiangsu Province "Collaborative Innovation Center for Advanced Industrial Fermentation" Industry Development Program, and First-Class Discipline Program of Light Industry Technology and Engineering (LITE2018-04).

语种：英文

外文关键词：Gene expression system; Auto-inducible; Nitrogen catabolite repression; Aspergillus nidulans

摘要：Background The construction of protein expression systems is mainly focused on carbon catabolite repression and quorum-sensing systems. However, each of these regulatory modes has an inherent flaw, which is difficult to overcome. Organisms also prioritize using different nitrogen sources, which is called nitrogen catabolite repression. To date, few gene regulatory systems based on nitrogen catabolite repression have been reported. Results In this study, we constructed a nitrogen switching auto-inducible expression system (NSAES) based on nitrogen catabolite regulation and nitrogen utilization in Aspergillus nidulans. The P-niaD promoter that is highly induced by nitrate and inhibition by ammonia was used as the promoter. Glucuronidase was the reporter protein. Glucuronidase expression occurred after ammonium was consumed in an ammonium and nitrate compounding medium, achieving stage auto-switching for cell growth and gene expression. This system maintained a balance between cell growth and protein production to maximize stress products. Expressions of glycosylated and secretory proteins were successfully achieved using this auto-inducible system. Conclusions We described an efficient auto-inducible protein expression system based on nitrogen catabolite regulation. The system could be useful for protein production in the laboratory and industrial applications. Simultaneously, NSAES provides a new auto-inducible expression regulation mode for other filamentous fungi.