文献类型：期刊文献

英文题名：Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum

作者：Zhao, Peng[1];Wu, Yuanyuan[2];Zhu, Yihua[1];Yang, Xiaoling[1];Jiang, Xin[1];Xiao, Jingfan[2];Zhang, Yuanxing[2];Li, Chunzhong[1]

机构：[1]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：6

期号：7

起止页码：3804

外文期刊名：NANOSCALE

收录：;EI(收录号：20141317504262);WOS:【SCI-EXPANDED(收录号:WOS:000333042500049)】；

基金：This work was supported by the National Natural Science Foundation of China (21236003, 21206042, 20925621, and 21176083), the Basic Research Program of Shanghai (13NM1400700, 13NM1400701), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Assays - Monoclonal antibodies

摘要：Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF4:Yb, Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified b-NaYF4:Yb, Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters. V. anguillarum was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10(2) CFU mL(-1), which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.