文献类型：期刊文献

英文题名：AIE-based nanoaggregate tracker: high-fidelity visualization of lysosomal movement and drug-escaping processes

作者：Liu, Zhenxing[1];Wang, Qi[1];Zhu, Zhirong[1];Liu, Ming[1];Zhao, Xiaolei[1];Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem,Sch Chem & Mol En, Key Lab Adv Mat,Feringa Nobel Prize Scientist Joi, Inst Fine Chem,Joint Int Res Lab Precis Chem & Mo, Shanghai 200237, Peoples R China

年份：2020

卷号：11

期号：47

起止页码：12755

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20205209690727);WOS:【SCI-EXPANDED(收录号:WOS:000599443300017)】；

基金：This work was supported by NSFC Science Center Program (21788102), NSFC Major Research Project (91959202), National Key Research and Development Program (2016YFA0200300), NSFC/China (21974047 and 21622602), Shanghai Pujiang Program (19PJ1402300), China Postdoctoral Science Foundation (2020M671328), Fundamental Research Funds for the Central Universities (222201814013), and Postdoctoral Science Foundation of Jiangsu Province (2020Z189).

语种：英文

外文关键词：Diagnosis - Visualization - Molecular biology

摘要：High-fidelity imaging and long-term visualization of lysosomes are crucial for their functional evaluation, related disease detection and active drug screening. However, commercial aggregation-caused quenching probes are not conducive to precise lysosomal imaging because of their inherent drawbacks, like easy diffusion, short emission and small Stokes shift, let alone their long-term tracing due to rapid photobleaching. Herein we report a novel aggregation-induced emission (AIE)-based TCM-PI nanoaggregate tracker for direct visualization of lysosomes based on the building block of tricyano-methylene-pyridine (TCM), wherein introduced piperazine (PI) groups behave as targeting units to lysosomes upon protonation, and the self-assembled nanostructure contributes to fast endocytosis for enhanced targeting ability as well as extended retention time for long-term imaging. The piperazine-stabilized TCM-PI nanoaggregate shifts the emission maximum to 677 nm in an aqueous environment, and falls within the desirable NIR region with a large Stokes shift of 162 nm, thereby greatly reducing biological fluorescent background interference. In contrast with the commercially available LysoTracker Red, the essential AIE characteristic of high photostability can guarantee three-dimensional high-fidelity tracing with low photobleaching, and little diffusion from lysosomes, and especially overcome the AIE bottleneck to target specificity. Consequently, the AIE-based nanoaggregate tracker successfully achieves the high-fidelity and long-term tracing of lysosomal movement and even monitors the drug-escaping process from lysosomes to cell nuclei, which provides a potential tool to benefit drug screening.