文献类型：期刊文献

中文题名：正丙醇、铜离子、烟酰胺对红霉素合成的作用

英文题名：Effects of Propanol,Cu^(2+) and Niacinamide on Biosynthesis of Erythromycin

作者：刘勇[1];叶蕊芳[1];郑黎[1];李晓勇[2];韩素芳[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]河南天方药业有限公司,河南驻马店463600

年份：2005

卷号：31

期号：6

起止页码：808

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2006049664200);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：红霉素;正丙醇;烟酰胺;铜离子;合成

外文关键词：erythromycin; propanol; propionyl-CoA synthetase; Cu^2＋; synthesis

摘要：在摇瓶培养过程中,发酵初期加入正丙醇可使红霉素的效价提高49.31%,正丙醇提高了丙酸激酶的比活力,表现出对该酶诱导作用,而对丙酰CoA合成酶比活力无影响。加入铜离子和烟酰胺可使红霉素效价分别提高24%和17%。用HPLC测定发酵液中有机酸含量,分析测定结果认为发酵过程可分为3个阶段:积累期、转折期和生产期。
Addition of propanol during erythromycin fermentation with in shake flask improved the titer by 49.3%. Propanol was found to increase the specific activity of propionate kinase, but it had no influence on the specific activity of propionyl-CoA synthetase. Addition of Cu^2＋ and niacinamide, increased erythromycin titer by 24% and 17%, respectively. Using HPLC, organic acid in the broth were measured; it was suggested that the fermentation process can be divided into three phases, accumulative phase, transitional phase and production phase. In production phase, the productivity of erythromycin was the highest.