文献类型：期刊文献

英文题名：Omics data reveal the unusual asexual-fruiting nature and secondary metabolic potentials of the medicinal fungus Cordyceps cicadae

作者：Lu, Yuzhen[1,2];Luo, Feifei[1,3];Cen, Kai[1,2];Xiao, Guohua[4];Yin, Ying[1];Li, Chunru[5];Li, Zengzhi[5];Zhan, Shuai[1];Zhang, Huizhan[3];Wang, Chengshu[1]

机构：[1]Chinese Acad Sci, CAS Key Lab Insect Dev & Evolutionary Biol, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[4]Fudan Univ, Sch Comp Sci, Shanghai 200433, Peoples R China;[5]Zhejiang BioAsia Inst Life Sci, Pinghu 314000, Peoples R China

年份：2017

卷号：18

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000408860300001)】；

基金：This work was supported by the National Natural Science Foundation of China (31530001) and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB11030100).

语种：英文

外文关键词：Cordyceps cicadae; Genomics; Mating type; Asexual fruiting; Secondary metabolism; Bioactive metabolites

摘要：Background: Ascomycete Cordyceps species have been using as valued traditional Chinese medicines. Particularly, the fruiting bodies of Cordyceps cicadae (syn. Isaria cicadae) have long been utilized for the treatment of chronic kidney disease. However, the genetics and bioactive chemicals in this fungus have been largely unexplored. Results: In this study, we performed comprehensive omics analyses of C. cicadae, and found that, in contrast to other Cordyceps fungi, C. cicadae produces asexual fruiting bodies with the production of conidial spores instead of the meiotic ascospores. Genome sequencing and comparative genomic analysis indicate that the protein families encoded by C. cicadae are typical of entomopathogenic fungi, including the expansion of proteases and chitinases for targeting insect hosts. Interestingly, we found that the MAT1-2 mating-type locus of the sequenced strain contains an abnormally truncated MAT1-1-1 gene. Gene deletions revealed that asexual fruiting of C. cicadae is independent of the MAT locus control. RNA-seq transcriptome data also indicate that, compared to growth in a liquid culture, the putative genes involved in mating and meiosis processes were not up-regulated during fungal fruiting, further supporting asexual reproduction in this fungus. The genome of C. cicadae encodes an array of conservative and divergent gene clusters for secondary metabolisms. Based on our analysis, the production of known carcinogenic metabolites by this fungus could be potentially precluded. However, the confirmed production of oosporein raises health concerns about the frequent consumption of fungal fruiting bodies. Conclusions: The results of this study expand our knowledge of fungal genetics that asexual fruiting can occur independent of the MAT locus control. The obtained genomic and metabolomic data will benefit future investigations of this fungus for medicinal uses.