文献类型：期刊文献

英文题名：Effect of porosity on long-term degradation of poly (ε-caprolactone) scaffolds and their cellular response

作者：Zhang, Qingchun[1];Jiang, Yun[2];Zhang, Yan[1];Ye, Zhaoyang[3];Tan, Wensong[3];Lang, Meidong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]Lib Zhejiang Univ Technol, Hangzhou 310023, Zhejiang, Peoples R China;[3]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：98

期号：1

起止页码：209

外文期刊名：POLYMER DEGRADATION AND STABILITY

收录：;EI(收录号：20130215882597);WOS:【SCI-EXPANDED(收录号:WOS:000315171000026)】；

基金：Fianacial support from Fundamental Research Funds for the Central Universities (WD0913008, WD1014036), the National Natural Science Foundation of China (20804015), "Shu Guang" Project of Shanghai Municipal Education Commission, Specialized Research Fund for the Doctoral Program of Higher Education (200802511021) and Program for Changjiang Scholars and Innovative Research Team in University (IRT0825)

语种：英文

外文关键词：Poly (epsilon-caprolactone); Scaffold; Degradation; Porosity; Cell culture

摘要：Porous poly(epsilon-caprolactone) (PCL) scaffolds are widely used as in vivo implants in tissue engineering, and their long-term degradation behaviors are of great importance for their in vivo performances. However, the influence of porosity on long-term degradation of PCL scaffold in phosphate buffer solution (PBS) has been rarely reported so far. Herein, a 72-week degradation study of PCL scaffolds with various porosities was conducted to elucidate the changes of physico-chemical properties such as weight, molecular weight, morphology and compressive modulus. Within 72 weeks, PCL scaffolds experienced three stages: stable stage, mechanical loss stage and structural collapse stage. The higher porosity induced the severer loss of weight, molecular weight and compressive modulus. It was found that a minimal acid autocatalysis also happened in the scaffold samples with low porosities (less than 85%). Cellular response on the scaffolds with various porosities was further evaluated. The cell ingrowth improved on the scaffold with high porosity (e.g. S-10) in contrast to those with low porosity (e.g. S-6 and S-4). The combined results demonstrated that an optimal porosity of PCL scaffolds should be designed greater than 90% due to the appropriate degradation rate and good cell performance. (C) 2012 Elsevier Ltd. All rights reserved.