文献类型：期刊文献

英文题名：Microscopic insight into the DNA condensation process of a zwitterion-functionalized polycation

作者：Sun, Hui[1,2,3];Zhou, Li[4];Chen, Xiaolu[2,3];Han, Xia[2,3];Wang, Rui[4];Liu, Honglai[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai 200237, Peoples R China

年份：2016

卷号：105

期号：11

起止页码：802

外文期刊名：BIOPOLYMERS

收录：;EI(收录号：20163402736217);WOS:【SCI-EXPANDED(收录号:WOS:000383370000005)】；

基金：The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (Nos. 21176065, 21376073, 91334203), the 111 Project of the Ministry of Education of China (No. B08021) and the Fundamental Research Funds for the Central Universities of China.

语种：英文

外文关键词：ATRP; DNA condensation; gene vector; PDMAEMA; sulfobetaine

摘要：Zwitterion-functionalized polycations are ideal gene carriers with long circulation, high cellular uptaking and low cell viability. However, the trade-off between the DNA condensation efficiency and the cell viability must be addressed. The purpose of this study is to provide a microscopic insight into the DNA condensation process and to explore the effect of a zwitterionic block of zwitterion-functionalized polycation, which is of great significance in designing novel gene delivery systems. Poly[2(dimethylamino) ethyl methacrylate-b-(sulfobetaine methacrylate)] (PDMAEMA-bPSBMA) copolymers were synthesized and used as the model systems. Different from the conventional concept that the PSBMA zwitterionic block act only as the "stealthy" groups, the subtle differences in physical and colloidal characteristics between the polycation/DNA polyplexes show that the PSBMA segment is capable of wrapping DNA attributed to the quaternary ammonium cations, without compromising the DNA condensation capability. On the other hand, the incorporation of PSBMA block reduces the surface charge of the polyplexes, which substantially result in the inefficient transfection and the reduced cytotoxicity.