文献类型：期刊文献

英文题名：Determination of the amino acid sequence in a cyclic lipopeptide using MS with DHT mechanism

作者：Yang, Shi-Zhong; Wei, Dong-Zhi; Mu, Bo-Zhong

机构：[1]E China Univ Sci & Technol, Sch Chem & Pharmaceut, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Bioengn, Shanghai 200237, Peoples R China

年份：2006

卷号：68

期号：1

起止页码：69

外文期刊名：JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000239185000007)】；

语种：英文

外文关键词：cyclic lipopeptide; TOF; MS; amino acid sequence; DHT

摘要：A TOF MS/MS method to directly determine the amino acid sequence in a cyclic lipopeptide without its hydrolysis is described. The fragments of the peptide and the hydrocarbon chains were identified through comparing the MS of two analogues of the lipopeptide: the connecting relationship of amino acid residues in the lipopeptide was determined based oil the difference of mass to charge ratio between peaks in the MS spectra and the amino acid analysis: and finally. according to the mechanism of double hydrogen transfer(DHT) the C-terminal of peptide and hydroxy aliphatic acid in the lipopeptide was directly determined without the hydrolysis. The determined sequence of amino acid residues in the cyclic lipopeptide is also supported by the rest peaks in the MS spectra grounded oil simple fragmenting mechanism. This method can be Used to determine the amino acid sequence in any aliphatic acid loop-in laying cyclic lipopeptides. (c) 2006 Elsevier B.V. All rights reserved.