文献类型：期刊文献

英文题名：Dual-Targeting Nanovesicles for In Situ Intracellular Imaging of and Discrimination between Wild-type and Mutant p53

作者：Qian, Ruocan[1];Cao, Yue[1];Long, Yi-Tao[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China

年份：2016

卷号：55

期号：2

起止页码：719

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20155001671873);WOS:【SCI-EXPANDED(收录号:WOS:000368069200046)】；

基金：This research was supported by the National Natural Science Foundation of China (21327807), the Science Fund for Creative Research Groups (21421004), the National Science Fund for Distinguished Young Scholars of China (21125522), and the China Postdoctoral Science Foundation (2015M570335).

语种：英文

外文关键词：dual targeting; fluorescence imaging; nanovesicles; plasmonic imaging; p53

摘要：p53 is a tumor-suppressor protein related to the cell cycle and programmed cell apoptosis. Herein, dual-targeting nanovesicles are designed for in situ imaging of intracellular wild-type p53 (WTp53) and mutant p53 (MUp53). Nanovesicle- encapsulated plasmonic gold nanoparticles (AuNPs) were functionalized with consensus DNA duplexes, and a fluorescein isothiocyanate (FITC)-marked anti-MUp53 antibody was conjugated to the nanovesicle surface. After entering the cytoplasm, the released AuNPs aggregated through recognition of WTp53 and the double-stranded DNA. The color changes of AuNPs were observed using dark-field microscopy, which showed the intracellular WTp53 distribution. The MUp53 location was detected though the immunological recognition between FITC-labeled anti-MUp53 and MUp53. Thus, a one-step incubation method for the in situ imaging of intracellular WTp53 and MUp53 was obtained; this was used to monitor the p53 level under a drug treatment.