文献类型：期刊文献

英文题名：Low-Noise Nanopore Enables In-Situ and Label-Free Tracking of a Trigger-Induced DNA Molecular Machine at the Single-Molecular Level

作者：Zhu, Zhentong[1,3];Duan, Xiaozheng[4];Li, Qiao[5];Wu, Ruiping[1,2];Wang, Yesheng[1,2];Li, Bingling[1,2]

机构：[1]Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China;[2]Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[4]Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China;[5]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：142

期号：9

起止页码：4481

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20201008255507);WOS:【SCI-EXPANDED(收录号:WOS:000518700800046)】；

基金：We are grateful to Network and Computing Center, CIAC, CAS, and Computing Center of Jilin Province for the essential support. We are grateful to Dr. Yi-Tao Long and Dr. Yi-Lun Ying at Nanjing University for providing the SiN nanopore. This work is supported by the K. C. Wong Education Foundation, Heilong Jiang Technology-based SME Technology Innovation Foundation (2017FK3GJ023), Company Cooperation Foundation of Heilongjiang Province (YS17c21), and National Natural Science Foundation of China (21404103).

语种：英文

外文关键词：Electrolytes - Amides - Noise abatement - Nanopores

摘要：Solid-state nanopores have shown special high potential in a label-free molecular assay, structure identification, and target-index at the single-molecular level, even though frustrating electrical baseline noise is still one of the major factors that limit the spatial resolution and signaling reliability of solid-state nanopores, especially in small target detection. Here we develop a significant and easy-operating noise-reduction approach via mixing organic solvents with high dielectric constants into a traditional aqueous electrolyte. The strategy is generally effective for pores made of different materials, such as the most commonly used conical glass (CGN) or SiNx. While the mechanism should be multisourced, MD simulations suggest the noise reduction may partially arise from the even ionic distribution caused by the addition of higher dielectric species. Among all solvents experimentally tested, the two with the highest dielectric constants, formamide and methylformamide, exhibit the best noise reduction effect for target detection of CGN. The power spectral density at the low-frequency limit is reduced by nearly 3 orders with the addition of 20% formamide. Our work qualifies the reliability of solid-state nanopores into much subtler scales of detection, such as dsDNAs under 100 bp. As a practical example, bare CGN is innovatively employed to perform in-situ tracking of trigger-responsive DNA machine forming oligomers.