文献类型：期刊文献

中文题名：利用交联杏仁粉作为廉价而稳定的催化剂通过优化逆水解反应体系显著提高长链烷基糖苷的平衡得率(英文)

英文题名：Significantly Improved Equilibrium Yield of Long-Chain Alkyl Glucosides via Reverse Hydrolysis in a Water-Poor System Using Cross-Linked Almond Meal as a Cheap and Robust Biocatalyst

作者：王琴琴[1];郁蕙蕾[1];赵娜[2];李春秀[1];尚亚卓[2];刘洪来[2];许建和[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]华东理工大学化学与分子工程系,上海200237

年份：2012

卷号：33

期号：2

起止页码：275

中文期刊名：催化学报

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：supported by the National Natural Science Foundation of China(20902023,31071604,and21173079);the Fundamental Research Funds for the Central Universities;the Foundation of Education Ministry(20090074120014);the National Key Technology R&D Program (2009BADB1B0301)~~

语种：中文

中文关键词：β-D-烷基葡萄糖苷;癸基β-D-葡萄糖苷;酶催化;逆水解;微水环境;平衡收率

外文关键词：alkyl β-D-glucosides; decyl β-D-glucoside; enzymatic synthesis; reverse hydrolysis; water-poor system; equilibrium yield

摘要：使用酶法合成了一系列不同烷基链长的β-D-烷基葡萄糖苷.结果表明,除甲基糖苷外,烷基碳链越长,反应初速度越低,糖苷的最终收率越低.从热力学角度系统地研究了这些糖苷合成的平衡常数和吉布斯自由能变化.通过改变反应的平衡,提高了长链烷基糖苷的最终收率.当水含量从10%降至5%(v/v),使用叔丁醇作为共溶剂,添加0.1mol/L葡萄糖作为底物时,癸基糖苷的平衡得率从1.9%提高至6.1%.除了其他长链的烷基糖苷外,庚基糖苷也显示了明显的表面活性剂活性.
An array of ten β-D-glucopyranosides with varied alkyl chain lengths were enzymatically synthesized.It was found that for longer alkyl chains a lower initial rate and final yield of glucoside was obtained except for methyl glucoside because of the severe toxicity of methanol to the enzyme.From a thermodynamics point of view,the equilibrium constant and Gibbs free energy variation of the glucoside syntheses were systematically investigated.To improve the final yields of the glucosides containing long alkyl chains the equilibrium of the enzymatic glucoside synthesis was altered.The equilibrium yield of decyl β-D-glucoside increased from 1.9% to 6.1% when the water content was reduced from 10% to 5%（v/v） using tert-butanol as a cosolvent and 0.10 mol/L of glucose as a substrate.As for the other longer alkyl chain glucosides,heptyl β-D-glucoside was found to have significant surface activity as well.