文献类型：期刊文献

英文题名：A novel hydrogen peroxide biosensor based on Au-graphene-HRP-chitosan biocomposites

作者：Zhou, Kangfu[1];Zhu, Yihua[1];Yang, Xiaoling[1];Luo, Jie[1];Li, Chunzhong[1];Luan, Shaorong[2]

机构：[1]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Ctr Anal & Test, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：55

期号：9

起止页码：3055

外文期刊名：ELECTROCHIMICA ACTA

收录：;EI(收录号：20100812730507);WOS:【SCI-EXPANDED(收录号:WOS:000276757500004)】；

基金：This work was supported by the National Natural Science Foundation of China (20676038 and 20976054), the Key Project of Science and Technology for Ministry of Education (107045), the Innovation Program of Shanghai Municipal Education Commission (09ZZ58), the Program of Shanghai Subject Chief Scientist (08XD1401500), the Shuguang Scholar-Tracking Foundation of Shanghai (08GG09), and the Shanghai Leading Academic Discipline Project (B502).

语种：英文

外文关键词：H2O2 biosensor; Graphene; Au nanoparticles; Horseradish peroxidase; Electrocatalyze

摘要：Graphene was prepared successfully by introducing -SO3- to separate the individual sheets. TEM, EDS and Raman spectroscopy were utilized to characterize the morphology and composition of graphene oxide and graphene. To construct the H2O2 biosensor, graphene and horseradish peroxidase (HRP) were co-immobilized into biocompatible polymer chitosan (CS), then a glassy carbon electrode (GCE) was modified by the biocomposite, followed by electrocleposition of Au nanoparticles on the surface to fabricate Au/graphene/HRP/CS/GCE. Cyclic voltammetry demonstrated that the direct electron transfer of HRP was realized, and the biosensor had an excellent performance in terms of electrocatalytic reduction towards H2O2. The biosensor showed high sensitivity and fast response upon the addition of H2O2, under the conditions of pH 6.5, potential -0.3 V. The time to reach the stable-state current was less than 3 s, and the linear range to H2O2 was from 5 x 10(-6) M to 5.13 x 10(-3) M with a detection limit of 1.7 x 10(-6) M (S/N = 3). Moreover, the biosensor exhibited good reproducibility and long-term stability. (C) 2010 Elsevier Ltd. All rights reserved.