文献类型：期刊文献

中文题名：超滤-纳滤技术在金毛狗脊黄酮分离纯化中的应用

英文题名：Application of ultrafiltration-nanofiltration technology in purification of flavonoids from Cibotium barometz

作者：韩伟[1];蒋一帅[1];魏雨恬[1]

机构：[1]华东理工大学药学院中药现代化研究中心,上海200237

年份：2015

卷号：37

期号：1

起止页码：1

中文期刊名：南京工业大学学报（自然科学版）

外文期刊名：Journal of Nanjing Tech University(Natural Science Edition)

收录：CSTPCD;;北大核心:【北大核心2014】；

语种：中文

中文关键词：超滤;纳滤;金毛狗脊;黄酮

外文关键词：ultrafihrafion ; nanofiltration ; Cibotium barometz; flavonoids

摘要：通过动态提取-超滤-纳滤操作分离金毛狗脊中的总黄酮。以去离子水为溶剂,在十二烷基硫酸钠(SDS)的辅助下动态循环提取金毛狗脊黄酮,并通过超滤去除大分子杂质,再用纳滤对料液进行浓缩。优化的工艺条件为:超滤操作压力0.14 MPa,温度45℃,溶液p H 6,操作时间90 min,此时超滤回收率61.30%,截留率18.52%;纳滤操作压力0.4 MPa,温度45℃,溶液p H 6,操作时间90 min,此时料液浓度提高2.190倍,纳滤回收率68.29%,截留率53.92%。与大孔树脂纯化实验相比,文中采用的分离方法能节省操作时间、提高操作效率,同时能有效提高总黄酮的浓度。
The dynamic extraction-uhrafiltration-nanofiltration operation was used to purify the flavonoids in Cibotium barometz. The flavonoids were extracted by the dynamic circulating water solution with the assistance of sodium dodecyl sulfate （SDS）, then the macromolecular impurities were removed by uhrafiltration and the feed was concentrated by nanofihration membrane finally.The ultrafihration yield of 61.30% and the retention rate of 18.52% were obtained under the following optimal conditions： ultrafihration pressure of 0. 14 MPa, temperature of 45 ℃, solution pH of 6, and operation time of 90 min. The nanofiltration yield reached 68.29% and the retention rate of 53.92% under nanofihration pressure of 0. 4 MPa, temperature of 45 ℃ , solution pH of 6, and operation time of 90 min with the trapped fluid flavonoid concentration raised to 2. 190 times than the original one. Compared with the nanofiltration and maeroporous resin purification, results showed that the succession operation method was time-saving and efficient, and the flavonoids was effectively concentrated.