文献类型：期刊文献

英文题名：Discovery of the non-cosmopolitan lineages in Candidatus Thermoprofundales

作者：Liu, Yi-Fan[1,2,3,4];Yang, Liu[1,2,3];Liu, Zhong-Lin[1,2,3];Chen, Jing[1,2,3];Fang, Bo[1,2,3];Zhou, Lei[1,2,3];Liu, Jin-Feng[1,2,3];Yang, Shi-Zhong[1,2,3];Gu, Ji-Dong[5];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr MEOR, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[5]Guangdong Techn, Israel Inst Technol, Environm Sci & Engn Grp, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China

年份：2022

卷号：24

期号：7

起止页码：3063

外文期刊名：ENVIRONMENTAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000803835800001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 42173076, 42061134011 and 52074129), the Natural Science Foundation of Shanghai (Grant No. 21ZR1417400), Fundamental Research Funds for the Central Universities (No. JKJ012016028) and the Research Program of State Key Laboratory of Bioreactor Engineering to Y.-F.L. and B.-Z.M, the NSFC/RGC Joint Research Fund (No. 41161160560) to J.-D.G.

语种：英文

摘要：The recently proposed order Candidatus Thermoprofundales, currently containing only one family-level lineage Marine Benthic Group-D (MBG-D), is distributed in global subsurface ecosystems and ecologically important, but its diversity, evolution and metabolism remain largely unknown. Here we described two novel family-level specialized lineages in Ca. Thermoprofundales, JdFR-43 and HyVt, which are restricted to specific biotopes (primarily in marine hydrothermal vents and occasionally in oil reservoirs and hot springs) in contrast to the cosmopolitan lineage MBG-D. The comparative genomics revealed that the specialized lineages have streamlined genomes, higher GC contents, enriched genes associated with nucleotide biosynthesis, ribosome biogenesis and DNA repair and additional thermostable aminopeptidases, enabling them to adapt to high-temperature habitats such as marine hydrothermal vents, deep subsurface oil reservoirs and hot springs. On the contrary, the unique metabolic traits of the cosmopolitan MBG-D, motility, glycolysis, butanoate metabolism, secondary metabolites production and additional genes for specific peptides and carbohydrates degradation potentially enhance its response to environmental change. Substrate preference is found for most MAGs across all lineages with the ability to utilize both polysaccharides (chitin and starch) and proteinaceous substances, whereas JdFR-43 members from oil reservoirs can only utilize proteins. These results expand the diversity of Ca. Thermoprofundales significantly and further improve our understandings of the adaptations of Ca. Thermoprofundales to various environments.