文献类型：期刊文献

英文题名：Research on preventive effect of Akkermansia muciniphila AKK PROBIO on acute gouty arthritis in mice

作者：Ma, Xin[1];Zhu, Na[2];Yu, Xueping[1];Wang, Wei[1];Wu, Wenzhong[3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chengdu Med Coll, Affiliated Hosp 1, Dept Anesthesia, Chengdu, Peoples R China;[3]Heilongjiang Red Cross Sengong Gen Hosp, Harbin 150040, Heilongjiang, Peoples R China

年份：2024

卷号：12

期号：10

起止页码：7644

外文期刊名：FOOD SCIENCE & NUTRITION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001282685800001)】；

基金：Suzhou Social Development Science and Technology Innovation Project, Grant/Award Number: 2022SS35

语种：英文

外文关键词：Akkermansia muciniphila; arthritis; expression; inflammation; oxidative stress

摘要：In mice with acute gouty arthritis, this study intends to examine the mechanism of action of Akkermansia muciniphila AKK PROBIO. We developed a mouse model of acute gouty arthritis using sodium urate. The efficiency and mechanism of AKK PROBIO in preventing acute gouty arthritis in mice were then determined by examining the degree of foot swelling, pain threshold, blood biochemical indicators, histological alterations, and messenger RNA (mRNA) expression changes. The results of the animal experiment showed that AKK PROBIO can lessen mouse foot edema severity and increase pain threshold. AKK PROBIO can enhance the enzyme activity of superoxide dismutase (SOD) and the level of glutathione (GSH) in the ankle joint tissues of mice with acute arthritis while decreasing the enzyme activity of myeloperoxidase (MPO) and the level of malondialdehyde (MDA). Interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) levels are all reduced by AKK PROBIO in the blood of mice with acute arthritis. Results from histopathology showed that AKK PROBIO reduced tissue damage in the mouse ankle and foot joints. In the tissues of the ankle joints of mice with acute arthritis, the results of the quantitative polymerase chain reaction (qPCR) and Western blot experiments suggested that AKK PROBIO may inhibit the mRNA and protein expression of extracellular signal-regulated kinase 1/2 (ERK1/2), cyclooxygenase-2 (COX-2), and prostaglandin E-2 (PGE(2)) in the tissues. AKK PROBIO can also regulate gut microbiota, inhibit harmful bacteria, and enhance valeric acid in the intestine, isobutyric acid, and isovaleric acid. Therefore, it is evident that AKK PROBIO prevents acute gouty arthritis better than glucosamine sulfate. It is a strain that has probiotic potential.