文献类型：期刊文献

英文题名：Decabromodiphenyl Ethane Mainly Affected the Muscle Contraction and Reproductive Endocrine System in Female Adult Zebrafish

作者：Sun, Yumiao[1,2];Zhu, Biran[1];Ling, Siyuan[3];Yan, Biao[1,2];Wang, Xiulin[1,2];Jia, Shuzhao[1];Martyniuk, Christopher J.[4,5];Zhang, Wei[3];Yang, Lihua[1];Zhou, Bingsheng[1]

机构：[1]Chinese Acad Sci, Inst Hydrobiol, Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Environm Risk Assessment & Control Chem P, Sch Resource & Environm Engn, Shanghai 200237, Peoples R China;[4]Univ Florida, Dept Physiol Sci, Genet Inst, Gainesville, FL 32611 USA;[5]Univ Florida, Interdisciplinary Program Biomed Sci Neurosci, Coll Vet Med, Gainesville, FL 32611 USA

年份：2022

卷号：56

期号：1

起止页码：470

外文期刊名：ENVIRONMENTAL SCIENCE & TECHNOLOGY

收录：;EI(收录号：20220111415003);WOS:【SCI-EXPANDED(收录号:WOS:000734064300001)】；

基金：This study was supported by the National Natural Science Foundation of China [grant numbers 21737005 and 42177268] and the State Key Laboratory of Freshwater Ecology and Biotechnology [grant number 2019FBZ03]. We would like to thank Min Wang of the Analysis and Testing Center of Institute of Hydrobiology for assistance in proteomic sample measurement.

语种：英文

外文关键词：decabromodiphenyl ethane; quantitative proteomics; parallel reaction monitoring; estrogenic response; muscle contraction

摘要：The novel brominated flame retardant decabromodiphenyl ethane (DBDPE) has become a widespread environmental pollutant. However, the target tissue and toxicity of DBDPE are still not clear. In the current study, female zebrafish were exposed to 1 and 100 nM DBDPE for 28 days. Chemical analysis revealed that DBDPE tended to accumulate in the brain other than the liver and gonad. Subsequently, tandem mass tag-based quantitative proteomics and parallel reaction monitoring verification were performed to screen the differentially expressed proteins in the brain. Bioinformatics analysis revealed that DBDPE mainly affected the biological process related to muscle contraction and estrogenic response. Therefore, the neurotoxicity and reproductive disruptions were validated via multilevel toxicological endpoints. Specifically, locomotor behavioral changes proved the potency of neurotoxicity, which may be caused by disturbance of muscular proteins and calcium homeostasis; decreases of sex hormone levels and transcriptional changes of genes related to the hypothalamic-pituitary-gonad-liver axis confirmed reproductive disruptions upon DBDPE exposure. In summary, our results suggested that DBDPE primarily accumulated in the brain and evoked neurotoxicity and reproductive disruptions in female zebrafish. These findings can provide important clues for a further mechanism study and risk assessment of DBDPE.