文献类型：期刊文献

英文题名：Amino-substituted C-coumarins: Synthesis, spectral characterizations and their applications in cell imaging

作者：Qiu, Shaobing[2];Dai, Dongdong[2];Guo, Chunlei[2];Sun, Zhenglong[3];Li, Hui[3];Qian, Xuhong[1,2];Yang, Youjun[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 20023Z, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, SIBET, Suzhou 215163, Jiangsu, Peoples R China

年份：2019

卷号：163

起止页码：55

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20184806160937);WOS:【SCI-EXPANDED(收录号:WOS:000457666300007)】；

基金：The work is financially supported by the National Natural Science Foundation of China (Nos. 21822805, 21572061, and 21236002).

语种：英文

外文关键词：Image processing - Spectrum analyzers

摘要：Compact fluorophores are desired for bioconjugation because they minimally perturb the native biological functions of proteins or organelles. However, their absorption and emission wavelengths are typically short and not ideal for practical applications. For example, coumarin is one of the smallest fluorophores with excellent fluorescence properties. However, its potentials in biological applications are limited by its relatively short excitation wavelengths in the UV spectral region. It is the aim of this manuscript to develop coumarin analogs absorbing and emitting in the visible range through rational molecular engineering. We designed and synthesized C-coumarin dyes, and found that their maximal absorption wavelengths fall well within the visible region, rendering them superior for live cell imaging than O-coumarins. The other promising properties of C-coumarins include larger Stokes' shifts, low cytotoxicity, and high chemostability.