文献类型：期刊文献

英文题名：Cyclophilin A Associates with Enterovirus-71 Virus Capsid and Plays an Essential Role in Viral Infection as an Uncoating Regulator

作者：Qing, Jie[1,2];Wang, Yaxin[2,3];Sun, Yuna[3];Huang, Jiaoyan[2];Yan, Wenzhong[4];Wang, Jinglan[2];Su, Dan[5,6];Ni, Cheng[7];Li, Jian[4];Rao, Zihe[2,3];Liu, Lei[1];Lou, Zhiyong[2,5,6]

机构：[1]Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ,Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China;[2]Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China;[3]Chinese Acad Sci, Inst Biophys, Natl Lab Macromol, Beijing 100080, Peoples R China;[4]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[5]Sichuan Univ, West China Med Sch, Collaborat Innovat Ctr Biotherapy, West China Hosp,State Key Lab Biotherapy, Chengdu 610064, Peoples R China;[6]Sichuan Univ, West China Med Sch, Ctr Canc, West China Hosp, Chengdu 610064, Peoples R China;[7]Beijing 4 High Sch, Beijing, Peoples R China

年份：2014

卷号：10

期号：10

外文期刊名：PLOS PATHOGENS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000344548800023)】；

基金：This work was supported by the Ministry of Science and Technology 973 (grant no. 2013CB911103 and 2014CB542800), the National Natural Science Foundation of China (grant nos. 81322023, 91313301, 31170678, 31370733 and 31000332), and The Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions (grant no. YETP0166). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Viruses utilize host factors for their efficient proliferation. By evaluating the inhibitory effects of compounds in our library, we identified inhibitors of cyclophilin A (CypA), a known immunosuppressor with peptidyl-prolyl cis-trans isomerase activity, can significantly attenuate EV71 proliferation. We demonstrated that CypA played an essential role in EV71 entry and that the RNA interference-mediated reduction of endogenous CypA expression led to decreased EV71 multiplication. We further revealed that CypA directly interacted with and modified the conformation of H-I loop of the VP1 protein in EV71 capsid, and thus regulated the uncoating process of EV71 entry step in a pH-dependent manner. Our results aid in the understanding of how host factors influence EV71 life cycle and provide new potential targets for developing antiviral agents against EV71 infection.