文献类型：期刊文献

英文题名：A collagen-based multicellular tumor spheroid model for evaluation of the efficiency of nanoparticle drug delivery

作者：Van-Minh Le[1];Lang, Mei-Dong[2];Shi, Wei-Bin[3];Liu, Jian-Wen[1]

机构：[1]E China Univ Sci & Technol, Biomed Nanotechnol Ctr, Sch Pharm,Shanghai Key Lab New Drug Design, Dept Mol & Cellular Pharmacol,State Key Lab Biore, 268 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat, Minist Educ, 268 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Dept Gen Surg, Shanghai 200030, Peoples R China

年份：2016

卷号：44

期号：2

起止页码：540

外文期刊名：ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY

收录：;EI(收录号：20161702308853);WOS:【SCI-EXPANDED(收录号:WOS:000370635000016)】；

基金：This work was supported by the Shanghai Committee of Science and Technology (No. 13140902300), Nano Science and Technology Special Funding of the Shanghai Committee of Science and Technology (No. 11nm0503700), the Shanghai Committee of Science and Technology [grant 11DZ2260600] and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：tissue engineering; drug delivery system; nanoparticles; three dimensional model; cancer

摘要：Targeted drug delivery systems, especially those that use nanoparticles, have been the focus of research into cancer therapy during the last decade, to improve the bioavailability and delivery of anticancer drugs to specific tumor sites, thereby reducing the toxicity and side effects to normal tissues. However, the positive antitumor effects of these nanocarriers observed in conventional monolayer cultures frequently fail in vivo, due to the lack of physical and biological barriers resembling those seen in the actual body. Therefore, the collagen-based 3-D multicellular culture system, to screen new nanocarriers for drug delivery and to obtain more adequate and better prediction of therapeutic outcomes in preclinical experiments, was developed. This 3-D culture model was successfully established using optimized density of cells. Our result showed that 3-D cell colonies were successfully developed from 95-D, U87 and HCT116 cell lines respectively, after a seven-day culture in the collagen matrix. The coumarin-conjugated nanoparticles were able to penetrate the matrix gel to reach the tumor cells. The model is supposedly more accurate in reflecting/predicting the dynamics and therapeutic outcomes of candidates for drug transport in vivo, and/or investigation of tumor biology, thus speeding up the pace of discovery of novel drug delivery systems for cancer therapy.