文献类型：期刊文献

英文题名：Direct synthesis of Cerium(Ⅳ)-Incorporated mesostructured cellular foam for immobilization of penicillin G acylase

作者：Liu, Yi[1,2]; Zhang, Haiyan[1]; Xu, Xuyang[1]; Zhu, Xiaoxiao[1]; Liu, Juan[3]; Ma, Liang[1]; Tian, Chengcheng[1,2]

机构：[1] National Engineering Laboratory for Industrial Wastewater Treatment, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China; [3] School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2021

卷号：312

外文期刊名：Microporous and Mesoporous Materials

收录：EI(收录号：20204809540714)

语种：英文

外文关键词：Hydrothermal synthesis - Silicon - Drug products

摘要：Cerium-incorporated mesostructured cellular foams (Ce-MCF) as a novel support has been synthesized successfully for immobilizing penicillin G acylase (PGA) through a Lewis acid-base interaction. The PGA was firmly immobilized on Ce-MCF by interacting with the surface robust Lewis acid sites due to the incorporation of Ce, which is the essence of this immobilization method. The Ce-MCF support was synthesized through the direct hydrothermal synthesis technique with citric acid complexant and categorized by XRD, SAXS, nitrogen sorption, XPS, TEM, and pyridine-IR. The results showed that the Ce-MCF samples had ordered long-range structure, large pore diameter, large specific surface area, pore-volume, narrow pore diameter distribution and strong Lewis acid site. According to the results, the PGA/Ce-MCF has high enzymatic activity and improved operational stability greatly. The PGA/Ce-MCF's initial enzymatic activity remains 10,573 U/g to hydrolyze penicillin G potassium salt, similar with that of PGA/Si-MCF. In addition, the Ce-MCF samples have much higher operational stability than PGA/Si-MCF. Followed by recycling for 10 times, PGA/Ce-MCF preserves 90% of its primary enzymatic activity, which is much higher than 77% of PGA/Si-MCF. ? 2020