文献类型：期刊文献

英文题名：Ultrasound assisted in situ separation of sophorolipids in multi-phase fermentation system to achieve efficient production by Candida bombicola

作者：Chen, Yang[1];Tang, Xu[1];Li, Ya[1];Liu, Chang[1];Zhuang, Yingping[1];Tian, Xiwei[1];Chu, Ju[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：17

期号：2

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20215011321593);WOS:【SCI-EXPANDED(收录号:WOS:000728697200001)】；

基金：This work was financially supported by the National Key Research Development Program of China (No. 2019YFA0904803). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：in situ separation; multiphase system; real-time; sophorolipids; ultrasonic enhanced sedimentation

摘要：Background Sophorolipids (SLs) are regarded as one of the most promising biosurfactants. However, high production costs are the main obstacle to extended SLs application. Semi-continuous fermentation, which is based on in situ separation, is a promising technology for achieving high SLs productivity. Methods and Results In this study, the sedimentation mechanism of SLs was analyzed. The formation of a hydrophobic mixture of SLs and rapeseed oil was a key factor in sedimentation. And the hydrophobicity and density of the mixture determined SLs sedimentation rate. On this basis, ultrasonic enhanced sedimentation technology (UEST) was introduced, by which the sedimentation rates were increased by 46.9%-485.4% with different ratio of rapeseed oil to SLs. UEST-assisted real-time in situ separation and semi-continuous fermentation were performed. SLs productivity and yield were 2.15 g L-1 h(-1) and 0.58 g g(-1), respectively, simultaneously the loss ratio of cells, glucose, and rapeseed oil were significantly reduced. Conclusions This study provides a new horizon for optimization of the SLs fermentation process.