文献类型：期刊文献

英文题名：Carbohydrate separation by hydrophilic interaction liquid chromatography on a 'click' maltose column

作者：Fu, Qing[1];Liang, Tu[2];Zhang, Xiuli[1];Du, Yuguang[1];Guo, Zhimou[1];Liang, Xinmiao[1,2]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China;[2]E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China

年份：2010

卷号：345

期号：18

起止页码：2690

外文期刊名：CARBOHYDRATE RESEARCH

收录：;EI(收录号：20104813429544);WOS:【SCI-EXPANDED(收录号:WOS:000285657700016)】；

基金：The authors gratefully acknowledge Dr Shuguang Li and Dr 31 Wenxia Wang (Natural Products and Glyco-biotechnology Dalian 32 Institute of Chemical Physics Chinese Academy of Sciences) for providing oligosaccharide samples This work was supported by a Project of the National Science Foundation of China (20805046 and 30801513) Major National Sci-Tech Projects (2009ZX09301-012) and the Foundation for Distinguished Young Scholars (No 20825518) from the National Natural Sciences Foundation of China

语种：英文

外文关键词：Oligosaccharides; HILIC; Click maltose; Separation; Purification

摘要：A general and efficient hydrophobic interaction liquid chromatography (HILIC) method has been developed for the separation of various kinds of carbohydrates ranging from galactooligosaccharides carrageenan oligosaccharides sodium alginate chitooligosaccharides to higher molecular weight fructooligosaccharides Three types of oligosaccharides (acidic basic and neutral) were purified by semi-preparative HILIC As a result 18 oligosaccharides in high purity were obtained and their degrees of polymerization ranged from DP 2 to 7 Furthermore oligosaccharides of plant origin were enriched and fractionated by solid-phase extraction (SPE) on a graphitized carbon cartridge The oligosaccharide fractions that eluted from the cartridge were purified and then characterized by ESIMS and NMR spectroscopy This strategy has led to the separation of a homogeneous raffinose family of oligosaccharides Since the present method is universal efficient and stable separation and purification of various types of carbohydrates by HILIC seem to be possible (C) 2010 Elsevier Ltd All rights reserved