文献类型：期刊文献

英文题名：Artificial cilia for soft and stable surface covalent immobilization of bone morphogenetic protein-2

作者：Gan, Qi[1,2];Chen, Lina[1];Bei, Ho-Pan;Ng, Sze-Wing[1];Guo, Han[4];Liu, Guoqiang[1];Pan, Hao[2];Liu, Changsheng[2];Zhao, Xin[3];Zheng, Zijian[1,5]

机构：[1]Hong Kong Polytech Univ, Sch Fash & Text, Lab Adv Interfacial Mat & Devices, Hong Kong Special Adm Reg China, Kowloon, Peoples R China;[2]East China Univ Sci & Technol, Sch Mat Sci & Engn, Key Lab Ultrafine Mat Minist Educ, Shanghai 200237, Peoples R China;[3]Hong Kong Polytech Univ, Dept Biomed Engn, Hong Kong, Peoples R China;[4]Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China;[5]Hong Kong Polytech Univ, Fac Sci, Dept Appl Biol & Chem Technol, Hong Kong Special Adm Reg China, Kowloon, Peoples R China

年份：2023

卷号：24

起止页码：551

外文期刊名：BIOACTIVE MATERIALS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000926632200001)】；

基金：The authors declare no conflict of interest. Zijian Zheng acknowledges financial support from RGC Senior Research Fellow Scheme (SRFS2122-5S04) , Research Grants Council of Hong Kong. Xin Zhao acknowledges Collaborative Research Fund (C5044-21GF) from the Research Grants Council of Hong Kong and National Excellent Young Scientists Fund (Hong Kong and Macau, 82122002) , National Natural Science Foundation of China. Qi Gan acknowledges support from National Natural Science Foundation of China (No. 32071337) and Shanghai Pujiang Program (20PJ1402600) .

语种：英文

外文关键词：Polymer brush; Poly (glycidyl methacrylate); Surface modification; Protein immobilization; Bioactivity

摘要：Preservation of growth factor sensitivity and bioactivity (e.g., bone morphogenetic protein-2 (BMP-2)) post-immobilization to tissue engineering scaffolds remains a great challenge. Here, we develop a stable and soft surface modification strategy to address this issue. BMP-2 (a model growth factor) is covalently immobilized onto homogeneous poly (glycidyl methacrylate) (PGMA) polymer brushes which are grafted onto substrate surfaces (Au, quartz glass, silica wafer, or common biomaterials) via surface-initiated atom transfer radical polymeri-zation. This surface modification method multiplies the functionalized interfacial area; it is simple, fast, gentle, and has little effect on the loaded protein owing to the cilia motility. The immobilized BMP-2 (i-BMP-2) on the surface of homogeneous PGMA polymer brushes exhibits excellent bioactivity (-87% bioactivity of free BMP-2 in vitro and 20%-50% higher than scaffolds with free BMP-2 in vivo), with conformation and secondary structure well-preserved after covalent immobilization and ethanol sterilization. Moreover, the osteogenic activity of i-BMP-2 on the nanoline pattern (PGMA-poly (N-isopropylacrylamide)) shows-110% bioactivity of free BMP-2. This is superior compared to conventional protein covalent immobilization strategies in terms of both bioactivity preservation and therapeutic efficacy. PGMA polymer brushes can be used to modify surfaces of different tissue-engineered scaffolds, which facilitates in situ immobilization of growth factors, and accelerates repair of a wide range of tissue types.