文献类型：期刊文献

英文题名：Efficient poly(3-hydroxybutyrate-co-lactate) production from corn stover hydrolysate by metabolically engineered Escherichia coli

作者：Wu, Ju[1];Wei, Xiangju[1];Guo, Pengye[1];He, Aiyong[4];Xu, Jiaxing[4];Jin, Mingjie[5];Zhang, Yanjun[3];Wu, Hui[1,2,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Zhejiang Normal Univ, Coll Chem & Life Sci, Inst Plant Genet & Dev Biol, Jinhua 321004, Zhejiang, Peoples R China;[4]Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian 223300, Peoples R China;[5]Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, 200 Xiaolingwei St, Nanjing 210094, Peoples R China;[6]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：341

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20213610874793);WOS:【SCI-EXPANDED(收录号:WOS:000696991200003)】；

基金：This study was funded by the National Natural Science Foundation of China (Grant No. 21776083) , the National Key Research and Development Program of China (Grant No.2017YFB0309302) , the Fundamental Research Funds for the Central Universities (Grant No. 22221818014) , the 111 Project (B18022) . Open foundation of Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology (BEETKC1801) and Huaian Science Foundation (HAB201702) . Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Poly(3-hydroxybutyrate-co-lactate); E; coli; Promoter of ldhA; Corn stover hydrolysate; Lactate fraction

摘要：Poly(3-hydroxybutyrate-co-lactate)[P(3HB-co-LA)], is a biodegradable and biocompatible bioplastic, and the monomeric composition of the copolymer plays an important role in affecting its mechanical properties. Corn stover hydrolysate (CSH), the waste by-product in agriculture, has been considered as an important carbon source for value-added biochemical production. Therefore, the effect of CSH on P(3HB-co-LA) biosynthesis was investigated in this study. Taking CSH as the carbon source, the lactate (LA) fraction in the copolymer reached 7.1 mol% by the engineered stain. The results of shake flask fermentation demonstrated that reducing the activity of electron transport system resulted in a higher LA fraction. Furthermore, we replaced the promoter of the key gene pctth with ldhA gene promoter, so that the expression of pctth gene could be dynamically modulated as well as the lactic acid content changed. This study suggests that CSH is a promising carbon source for the production of biodegradable P(3HB-co-LA).