文献类型：期刊文献

英文题名：Soft-template synthesis of hydrophilic metallic zirconia nanoparticle-incorporated ordered mesoporous carbon composite and its application in phosphopeptide enrichment

作者：Zhang, Lei[1];Xiong, Zhichao[2];Chen, Yajing[1];Peng, Li[1];Yu, Bohao[1];Gao, Xiaodi[1];Zhang, Runsheng[3];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China;[3]Shanghai Publ Secur Bur, Shanghai Res Inst Criminal Sci & Technol, Shanghai Key Lab Crime Scene Evidence, Shanghai, Peoples R China

年份：2016

卷号：6

期号：36

起止页码：30014

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20190106346021);WOS:【SCI-EXPANDED(收录号:WOS:000373061600017)】；

基金：This work was financially supported by the National 973 Program (2011CB910403), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the National Natural Science Foundation of China (21475044) and foundation of Shanghai Research Institute of Criminal Science and Technology.

语种：英文

外文关键词：Carbon carbon composites - Metals - Affinity chromatography - Synthesis (chemical) - Carbonization - Hydrophilicity

摘要：Novel nanostructure metallic zirconia incorporated ordered mesoporous carbon (OMC) composites were synthesized via a multi-component co-assembly strategy associated with a direct carbonization process from resol, ZrO(NO3)(2)center dot xH(2)O, and triblock copolymer F127. Herein, the novel metallic zirconia incorporated OMC as a Metal Oxide Affinity Chromatography (MOAC) material had large mesopores (5.6 nm), a high surface area (387 m(2) g(-1)), a large pore volume (0.35 cm(3) g(-1)) and excellent hydrophilicity for the selective enrichment of phosphopeptides. The surface construction and chemical composition of the material were characterized by SEM, TEM, XPS, EDS, BET, and XRD. Experimental conditions for trapping phosphopeptides were optimized using beta-casein as the standard protein. Under the optimal conditions combined with the merits of high surface area of material and special combination with phosphopeptides, the zirconia/OMC composites for enrichment of the phosphopeptides showed a high selectivity (phosphopeptides/non-phosphopeptides at a molar ratio of 1 : 300) and lower detection sensitivity (1.5 fmol). Moreover, the as-prepared MOAC zirconia/OMC composites were successfully applied to the detection and identification of low-abundance phosphopeptides from non-fat milk.