文献类型：期刊文献

英文题名：A hybrid erbium(III)-bacteriochlorin near-infrared probe for multiplexed biomedical imaging

作者：Wang, Ting[1,2];Wang, Shangfeng[1,2];Liu, Zhiyong[3];He, Zuyang[1,2];Yu, Peng[1,2];Zhao, Mengyao[1,2];Zhang, Hongxin[1,2];Lu, Lingfei[1,2];Wang, Zhengxin[4];Wang, Ziyu[4];Zhang, Weian[3];Fan, Yong[1,2];Sun, Caixia[1,2];Zhao, Dongyuan[1,2];Liu, Weimin[4];Bunzli, Jean-Claude G.[5];Zhang, Fan[1,2]

机构：[1]Fudan Univ, Dept Chem, State Key Lab Mol Engn Polymers, Shanghai Key Lab Mol Catalysis & Innovat Mat, Shanghai, Peoples R China;[2]Fudan Univ, iChem, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai, Peoples R China;[4]ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China;[5]Ecole Polytech Fed Lausanne, Inst Sci Chim & Ingn, Lausanne, Switzerland

年份：2021

卷号：20

期号：11

起止页码：1571

外文期刊名：NATURE MATERIALS

收录：;EI(收录号：20213110722732);WOS:【SCI-EXPANDED(收录号:WOS:000679300200003)】；

基金：We thank Xuan Zhang (Techcomp), Mi Wang (Techcomp), Xiaolei Zuo (Shanghai Renji Hospital) and Fangfei Yin (Shanghai Renji Hospital) for their help with absolute quantum yield measurements. F.Z. and D.Z. acknowledge support by the National Key R&D Program of China (grant no. 2017YFA0207303) and the National Natural Science Foundation of China (NSFC, grant nos. 22088101 and 51961145403). F.Z. also acknowledges support by the NSFC (grant no. 21725502) and the Research Program of the Science and Technology Commission of Shanghai Municipality (grant no. 20JC1411700). S.W. acknowledges support by the NSFC (grant no. 22004018) and the China Postdoctoral Science Foundation (grant nos. KLH1615200 and KLH1615215). Y.F. acknowledges support by the NSFC (grant no. 21904023) and the Research Program of the Science and Technology Commission of Shanghai Municipality (grant no. 19490713100). H.Z. acknowledges support by the Research Program of the Science and Technology Commission of Shanghai Municipality (grant no. 20490710600). J.-C.G.B. acknowledges support through a senior visiting scholarship (grant no. 20FGJ04) at the State Key Laboratory of Molecular Engineering of Polymers.

语种：英文

外文关键词：Forster resonance energy transfer - Fluorophores - Probes - Erbium compounds - Infrared devices - Medical imaging - Energy transfer

摘要：Spectrally distinct fluorophores are desired for multiplexed bioimaging. In particular, monitoring biological processes in living mammals needs fluorophores that operate in the 'tissue-transparent' near-infrared (NIR) window, that is, between 700 and 1,700 nm. Here we report a fluorophore system based on molecular erbium(III)-bacteriochlorin complexes with large Stokes shift (>750 nm) and narrowband NIR-to-NIR downconversion spectra (full-width at half-maximum <= 32 nm). We have found that the fast (2 x 10(9) s(-)(1)) and near-unity energy transfer from bacteriochlorin triplets to the erbium(III) I-4(13/2) level overcomes the notorious vibrational overtones quenching, resulting in bright and long-lived (1.73 mu s) 1,530 nm luminescence in water. We demonstrate the excitation/emission-multiplexed capability of the complexes in the visualization of dynamic circulatory and metabolic processes in living mice, and through skull tracking of cancer cell metastases in mouse brain. This hybrid probe system facilitates robust multiplexed NIR imaging with high contrast and spatial resolution for applications ranging from fluorescence-guided surgery, diagnostics and intravital microscopy. An erbium(III)-bacteriochlorin probe with large Stokes shift and efficient near-infrared to near-infrared energy conversion enables multiplexed imaging of deep tissues in living animals.