文献类型：期刊文献

英文题名：Quaternized chitosan oligomers as novel elicitors inducing protection against B. cinerea in Arabidopsis

作者：Feng, Huafeng[1];Xia, Wei[1];Shan, Chi[2];Zhou, Tingting[1];Cai, Weiming[1,2];Zhang, Wenqing[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China

年份：2015

卷号：72

起止页码：364

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20240815604330);WOS:【SCI-EXPANDED(收录号:WOS:000347577900049)】；

基金：This work was supported by the Strategic Priority Research Program on Space Science, the Chinese Academy of Sciences, China (grant no. XDA04020202-15); the National Basic Research Program of China, China (grant no. 2011CB710902); the National Scientific Program, China (grant no. 2012AA101103-04), the National Natural Science Foundation of China, China (grant no. 31070237) and the China Manned Space Flight Technology Project, China.

语种：英文

外文关键词：Quaternized chitosan oligomers; B. cinerea; Plant defense elicitors

摘要：Chitosan oligomers prepared from enzyme hydrolysis of chitosan have for many years been recognized as potent elicitors of plant innate immunity, but their efficacy is limited by the degree of polymerization and the degree of acetylation. In this study, we presented a new type of chitosan oligomers (COS), with the name of quaternized chitosan oligomers (QCOS) that were prepared by reacting COS with glycidyltrimethylammonium chloride and overcome these problems. First, QCOS clearly induced hydrogen peroxide accumulation and callose deposition in Arabidopsis seedlings. Second, we found that PAD3 expression was significantly upregulated more than 5-fold by QCOS as compared to COS. Further, PAD3 expression activated by QCOS was required for inducing Arabidopsis resistance to the necrotrophic fungus Botrytis cinerea, independent of salicylic acid signaling. These results demonstrate that quaternized modifications of COS possess better elicitor properties than the original COS and that QCOS stimulate plant protection against B. cinerea attack in Arabidopsis. Importantly, our work provides a novel and valuable strategy for enhancing elicitor activities of other types of oligosaccharides for plant innate immunity. (C) 2014 Elsevier B.V. All rights reserved.