文献类型：期刊文献

中文题名：重组人Cu·Zn-SOD工程菌发酵工艺的研究

英文题名：Studies on Fermentative Technology of Human Cu·Zn-SOD in E. coli

作者：陈长华[1];付水林[1];范立强[1];苏惠娟[1];魏东芝[1];袁勤生[1]

机构：[1]华东理工大学生物工程学院,上海200237

年份：1999

卷号：6

期号：3

起止页码：135

中文期刊名：药物生物技术

外文期刊名：Pharmaceutical Biotechnology

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：SOD;发酵;重组;大肠杆菌;铜;锌

外文关键词：Superoxide dismutase, Fermentation, Recombinant E. coli

摘要：人Cu·Zn SOD是一种重要的氧自由基清除剂 ,传统工艺是从动物血中提取得到 ,受原料来源的限制。从人胎肝中提取了hCu·Zn SODcDNA构建了基因工程菌 ,同时对其表达产物的条件进行研究 ,着重探索了接种量、接种时机、pH、溶氧等因素 ,并优化了发酵条件。通过在 5L全自动发酵罐上研究重组菌BL2 1的培养条件 ,确定了最适条件为 :接种量 2 % ,溶氧水平≥ 40 % ,初始 pH6 5 ,培养过程中用 3mol/LHCl调节 pH在 7 5。在此条件下 ,发酵最高水平达到酶活95 0U/ml,比活 14 0 0U/mg。
Human Cu·Zn SOD is an important oxygen free radical scavenger In the traditional technology, it was extracted from animal blood of which the resources were limited In this experiment, hCu·Zn SOD cDNA was extracted from foetal liver to form an recombinant E coli and the conditions of product expression were also studied ,such as inoculum quantities, inoculum time, pH, dissolved oxygen, Then the suitable technology was obtained Being studied in 5L fermentor, the optimal technology was found :inoculum 2%, dissolved oxygen≥40%, primary pH 6 5, regulating pH to 7 5 by 3mol/L HCl during the process of culture Under these conditions, the maximum expression of enzyme activity reached 950U/ml and the maximum specific activity reached 1 400U/mg