文献类型：期刊文献

英文题名：An alternative s factor sLsl regulates lincomycin production in Streptomyces lincolnensis

作者：Tu, Bingbing[1];Mao, Yue[1];Wang, Ruida[1,2];Kang, Yajing[1];Ye, Jiang[1,2];Zhang, Huizhan[1,2];Wu, Haizhen[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：63

期号：2

起止页码：190

外文期刊名：JOURNAL OF BASIC MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000892917000001)】；

语种：英文

外文关键词：alternative sigma factor; lincomycin; Streptomyces lincolnensis; sigma(L)(sl)

摘要：Lincomycin produced by Streptomyces lincolnensis is a critical antibacterial antibiotic in the clinical. To further understand the regulatory mechanism of lincomycin biosynthesis, we identified an alternative sigma factor, sigma(L)(sl), in Streptomyces lincolnensis NRRL 2936. Deletion of sigL(sl) resulted in an increase in cell growth but a decrease in lincomycin production. sigma(L)(sl) boosted lincomycin biosynthesis by directly stimulating the transcription of four genes (lmbD, lmbV, lmrC, and lmbU) within the lincomycin biosynthetic lmb gene cluster. Besides, sigma(L)(sl) participated in lincomycin biosynthesis by directly stimulating the transcription of mshC, a gene responsible for MSH synthesis. In conclusion, our findings demonstrated that sigma(L)(sl) plays a direct regulatory role in lincomycin biosynthesis. This study extends the understanding of molecular mechanisms of lincomycin biosynthetic regulation.