文献类型：期刊文献

英文题名：Analysis of vibrational dynamics in cell-substrate interactions using nanopipette electrochemical sensors

作者：Gong, Li -Juan[1];Lv, Jian[1];Wang, Xiao-Yuan[1];Wu, Xue[1];Li, Da -Wei[1];Qian, Ruo-Can[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn, Joint Res Ctr,Key Lab Adv Mat,Joint Key Lab Adv Ma, Shanghai 200237, Peoples R China

年份：2024

卷号：259

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20242016099775);WOS:【SCI-EXPANDED(收录号:WOS:001242533900001)】；

基金：This research was supported by National Natural Science Foundation of China (22176058) , the China Postdoctoral Science Foundation (2021M701195) , Shanghai Science and Technology Committee (19520744000, 22ZR1416800, 23ZR1416100) , the Program of Introducing Talents of Discipline to Universities (B16017) and the Fundamental Research Funds for the Central Universities (222201717003) . The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：Nanopipettes; Cell-substrate; Cell membrane; Mechanical status; Single-cell

摘要：Cell -substrate interaction plays a critical role in determining the mechanical status of living cell membrane. Changes of substrate surface properties can significantly alter the cell mechanical microenvironment, leading to mechanical changes of cell membrane. However, it is still difficult to accurately quantify the influence of the substrate surface properties on the mechanical status of living cell membrane without damage. This study addresses the challenge by using an electrochemical sensor made from an ultrasmall quartz nanopipette. With the tip diameter less than 100 nm, the nanopipette-based sensor achieves highly sensitive, noninvasive and label -free monitoring of the mechanical status of single living cells by collecting stable cyclic membrane oscillatory signals from continuous current versus time traces. The electrochemical signals collected from PC12 cells cultured on three different substrates (bare ITO (indium tin oxides) glass, hydroxyl modified ITO glass, amino modified ITO glass) indicate that the microenvironment more favorable for cell adhesion can increase the membrane stiffness. This work provides a label -free electrochemical approach to accurately quantify the mechanical status of single living cells in real-time, which may help to better understand the relationship between the cell membrane and the extra cellular matrix.