文献类型：期刊文献

英文题名：Universal DNA-Based Sensing Toolbox for Programming Cell Functions

作者：Ma, Pei-Qiang[1];Huang, Fu-Wen[1];Xie, Ya-Qi[1];Li, Hong-Rui[1];Li, Hua-Dong[1];Ye, Bang-Ce[1,2];Yin, Bin-Cheng[1,3]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Inst Engn Biol & Hlth, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832000, Xinjiang, Peoples R China

年份：2023

卷号：145

期号：51

起止页码：28224

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20235215293463);WOS:【SCI-EXPANDED(收录号:WOS:001133416800001)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (grants 22134003, 22374047, 22104037) and the Fundamental Research Funds for the Central Universities. We thank Prof. Yangyang Yang and Dr. Yue Liao (School of Pharmacy, East China University of Science and Technology) for their assistance with origami framework design and experimental guidance and Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization experiments.

语种：英文

外文关键词：Bacteria - Cell death - Cell engineering - Cell membranes - Cell signaling - Diagnosis

摘要：By recombining natural cell signaling systems and further reprogramming cell functions, use of genetically engineered cells and bacteria as therapies is an innovative emerging concept. However, the inherent properties and structures of the natural signal sensing and response pathways constrain further development. We present a universal DNA-based sensing toolbox on the cell surface to endow new signal sensing abilities for cells, control cell states, and reprogram multiple cell functions. The sensing toolbox contains a triangular-prismatic-shaped DNA origami framework and a sensing core anchored inside the internal confined space to enhance the specificity and efficacy of the toolbox. As a proof of principle, the sensing toolbox uses the customizable sensing core with signal sensing switches and converters to recognize unconventional signal inputs, deliver functional components to cells, and then control cell responses, including specific tumor cell death, immune cell disinhibition and adhesion, and bacterial expression. This work expands the diversity of cell sensing signals and reprograms biological functions by constructing nanomechanical-natural hybrid cells, providing new strategies for engineering cells and bacteria in diagnosis and treatment applications.