文献类型：期刊文献

英文题名：Isolation of a Bacillus strain producing ketone reductase with high substrate tolerance

作者：Xie, Yan[1]; Xu, Jian-He[1]; Xu, Yi[1]

机构：[1] Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2010

卷号：101

期号：3

起止页码：1054

外文期刊名：Bioresource Technology

收录：EI(收录号：20095012535167)

语种：英文

外文关键词：Bacteriology - Aromatic compounds - Ethanol - Ketones

摘要：Target reaction-oriented screening from soil samples yielded a ketone reductase-producing Bacillus sp., strain ECU0013, which exhibits excellent stereoselectivity, high substrate tolerance and is capable of regenerating the required cofactor with glucose as a co-substrate. Whole-cells catalyzed the asymmetric reduction of 2-chloro-1-phenylethanone (50 mM) to (R)-2-chloro-1-phenylethanol with a 93.3% conversion rate and 99% e.e. (enantiomeric excess). A variety of ketones were enantioselectively reduced by resting cells, giving corresponding chiral alcohols with good to excellent e.e. values. These results suggest the potential of this strain for the industrial production of chiral halogenated aromatic alcohols. ? 2009 Elsevier Ltd. All rights reserved.