文献类型：期刊文献

英文题名：Switch-on mode of bioenergetic channels regulated by curcumin-loaded 3D composite scaffold to steer bone regeneration

作者：Wei, Jianxu[1,2,3,4,5];Zhang, Xiaomeng[1,2,3,4,5];Zhang, Zhuo[1,2,3,4,5];Ding, Xinxin[1,2,3,4,5];Li, Yuan[1,2,3,4,5];Zhang, Yi[1,2,3,4,5];Jiang, Xue[1,2,3,4,5];Zhang, Hongbo[6];Lai, Hongchang[1,2,3,4,5];Shi, Junyu[1,2,3,4,5]

机构：[1]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Oral Implantol, Sch Med, Shanghai, Peoples R China;[2]Shanghai Jiao Tong Univ, Coll Stomatol, Shanghai, Peoples R China;[3]Natl Ctr Stomatol, Shanghai, Peoples R China;[4]Natl Clin Res Ctr Oral Dis, Shanghai, Peoples R China;[5]Shanghai Key Lab Stomatol, Shanghai, Peoples R China;[6]East China Univ Sci & Technol, Sch Mech & Power Engn, Shanghai, Peoples R China

年份：2023

卷号：452

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20223812768792);WOS:【SCI-EXPANDED(收录号:WOS:000863429300002)】；

基金：We thank Mr. Tiandi Wang for improving the 3D printing protocol ofthe scaffolds. This work was supported by the National Natural Science Foundation of China (82171005, 82001098) ; the Project of the Funda-mental Research Program Funding of Ninth People?s Hospital, Shanghai Jiao Tong University School of Medicine (JYZZ102) ; the Innovative Research Team of High-Level Local Universities in Shanghai (SHSMU-ZDCX20212500) ; the Natural Science Foundation of Shanghai ( [2020] 20ZR1431700) .

语种：英文

外文关键词：Curcumin; Lipid raft; GLUT1; Bone regeneration; Switch

摘要：Metabolic energy to steer osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) could be a promising therapeutic target for bone tissue engineering (BTE), but prior knowledge of this issue is limited. To address bone defects with BTE, we customized a three-dimensional (3D)-printed composite scaffold (Cur@MS) to allow the controlled release of curcumin, which could facilitate the "switch-on" mode of Glucose transporter 1 (GLUT1) in BMSCs. Consequently, bioenergetic channels, i.e. glucose uptake, were "switched on" to activate GLUT1-RUNX2 crosstalk, which was closely orchestrated with bone regeneration. Furthermore, curcumin-induced cholesterol/lipid raft (Cho/LR) was a "sensor" to trigger the "switch" (GLUT1) by directing its spatial distribution into clusters. In contrast, selective inhibition of Cho/LR and GLUT1 led to a "switch-off" mode and compromised bone regeneration in vivo. Overall, the results suggest Cho/LR is a potential target to steer BMSCs and Cur@MS is an ideal BTE material for stimulating rapid bone regeneration.