文献类型：期刊文献

中文题名：钼对红霉素生物合成的影响

英文题名：Influence of Molybdenum on Biosynthesis of Erythromycin

作者：李啸[1];陈长华[1];李友元[1];涂志英[2]

机构：[1]华东理工大学国家生物反应器重点实验室,上海200237;[2]三峡大学化学与生命科学学院,湖北宜昌443002

年份：2006

卷号：28

期号：4

起止页码：355

中文期刊名：三峡大学学报（自然科学版）

外文期刊名：Journal of China Three Gorges University：Natural Sciences

收录：CSTPCD

基金：河南省第三批科技发展计划项目(05230511300)

语种：中文

中文关键词：红霉素;糖多孢红霉菌;酶活;有机酸;钼酸钠

外文关键词：erythromycin; Saccharopolyspora erythraea; enzyme activity; organic acid; molybdenum acid sodium

摘要：在利用糖多孢红色链霉菌HB发酵生产红霉素的过程中,于48 h向发酵液中加入钼酸钠,通过对代谢途径关键调控酶活力的测定以及运用高效液相色谱法对发酵液中相关有机酸含量的测定,结论显示:当发酵液中钼酸钠的加入量为0.056 g/100 mL时,部分代谢流向有利于红霉素生物合成的方向迁移,在一定程度上提高了红霉素的生产速率和产量.
During the process of utilizing Saccharopolyspora erythraea HB to ferment and produce erythromytin, add Na2MoO4 to the fermented broth after the fermented course lasts 48 hours. Adopt to the determination of the activity of some key regulatory enzymes in the metabolic pathway, and HPLC was utilized to determine relevant organic acid content of the fermented broth. The results show that.when the adding amount of Na2MoO4 is 0. 056 g/100 mL in the fermented broth, some metabolic fluxes move to the routes favorable to the biosynthesis of erythromycin; to some extent,we have raised the production speed and output of erythromycin.