文献类型：期刊文献

中文题名：淀粉／PEG制备双孔块状SiO_2载体材料及其酶活力

英文题名：Synthesis and Enzyme Activity of SiO_2 Monolithic Carrier with Double-pore Structure by Addition of Starch/PEG

作者：杨洪斌[1];陈奇[1];宋鹂[1];叶蕊芳[2];候凤珍[1];陆剑英[1]

机构：[1]华东理工大学无机材料系,上海200237;[2]华东理工大学生物工程系,上海200237

年份：2006

卷号：21

期号：5

起止页码：1154

中文期刊名：无机材料学报

外文期刊名：Journal of Inorganic Materials

收录：CSTPCD;;EI(收录号：20064810278276);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：双孔结构;多孔块体;溶胶-凝胶法;酶载体

外文关键词：double-pore structure; porous monolithic; sol-gel; enzyme carrier

摘要：采用溶胶-凝胶法,以聚乙二醇为介孔造孔剂、淀粉为大孔造孔剂,经过600℃热处理,制得介孔平均孔径为10nm左右、大孔平均孔径为8-11μm的SiO2块状材料．样品的最小密度为0．34g·cm-3,最大气孔率为76％．引入30％淀粉制备多孔样品作固定葡萄糖淀粉酶的载体,可使初始酶活力由原来的5994U提高至14702U．且连续使用五次之后仍具有一定的酶活力．另外在80℃水中浸泡7天之后,多孔样品对酶的吸附量均有提高．浸泡前后,酶活力相差不大．这些结果表明:此类多孔材料在固定葡萄糖淀粉酶方面可长时间使用,便于酶的回收、保存和再利用．
SiO2 monoliths with double-pore structure were successfully synthesized with polyethylene glycol as mesopore-making agent and starch as macropore-making agent via a sol-gel route. The porous blocks, having mesopores with about 10nm pore size and macropores with 8μm pore size, could be formed from the removal of polyethylene glycol and starch after heated at 600℃. The minimal density of the obtained samples was 0.34g·cm^-3 and the maximal porosity was 76%. The original enzyme activity was improved from 5994U to 14702U by adding 30% of starch to the bare SiO2 sample. After immersed in water at 80℃ for 7 days, the enzyme adsorption of the samples was increased and there was little difference between enzyme activity before and after immersion. Those results indicated that such double-pore materials with longterm stability on gluczyme immobilization should be easy to reclamation, reservation and recycle of enzyme.