文献类型：期刊文献

中文题名：血清中的氨基酸及蛋白质降解产物的FDMS研究

英文题名：Field Desorption Mass spectrometric Investigation of the Amino Acid and Protein Degraded Products in Serum

作者：诸培奋[1];张锐[1];张明静[1];俞文(鱼比)[2];黄颂羽[1]

机构：[1]华东化工学院分析中心;[2]华东化工学院数学系

年份：1993

卷号：14

期号：1

起止页码：54

中文期刊名：质谱学报

外文期刊名：Journal of Chinese Mass Spectrometry Society

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：血清;氨基酸;蛋白;场解吸质谱

外文关键词：serum, amino acid, protein degraded products, FDMS

摘要：用FDMS方式对经预处理后的人体静脉血样中的血清进行测试,获得血样中的混合氨基酸及肽的分子量分布。实验还表明增加血样的酸浓度及水解时间,血样的FDMS棒图则向小分子量方向移动,反之则向大质量数移动;最多测得六肽;对应所得到的质荷比值,列出了部分可能的氨基酸组成。并且还对血清中加入甲醇、乙醇的血样做了测定,对实验结果进行了初步解释。
Samples of pretreated serum from human-body's vein were analysed by field desorption mass spectrometry (FDMS). The molecular weight distribution of mixing amino acids and peptides from serum was obtianed. The experimental results showed when acid concentration in serum sample was increased and hydrolysis time was prolonged that the bar graphs form of a spectrum were moved to small masses, on the contrary, to large masses. The most six peptides were got. Parts of possible component of amino acids into correspondence with mass numbers were kisted. Furthermore, serum which was added ethanol or methanol was determined, and the experimental results were explained initially.