文献类型：期刊文献

英文题名：Electrostatic-DrivenCharge Convertible Exosome NanoclusterSelf-Assembly for Enhanced Cellular Uptake and Vascular Injury Therapy

作者：Yuan, Chen[1];Wu, Yue[1,2,3,4];Lu, Shan[5,6,7];Cai, Minghao[5,6,7];Wang, Ruihan[5,6,7];Yu, Miaojie[2,3,4];Li, Jiaxiong[1];Guo, Zhiqian[2,3,4];Si, Yi[5,6,7];Xu, Yisheng[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, State Key Lab Chem Engn & Low Carbon Technol, State Key Lab Green Chem Engn & Ind Catalysis, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Ctr Photosensit Chem Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Inst Fine Chem,Frontiers Sci Ctr Materiobiol & Dyn, Sch Chem & Mol Engn,Feringa Nobel Prize Scientist, Shanghai Key Lab Funct Mat Chem,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Inst Fine Chem,Frontiers Sci Ctr Materiobiol & Dyn, Sch Chem & Mol Engn,Feringa Nobel Prize Scientist, Joint Int Res Lab Precis Chem & Mol Engn, Shanghai 200237, Peoples R China;[5]Fudan Univ, Zhongshan Hosp, Dept Vasc Surg, Shanghai 200032, Peoples R China;[6]Fudan Univ, Inst Vasc Surg, Shanghai 200032, Peoples R China;[7]Natl Clin Res Ctr Intervent Med, Shanghai 200032, Peoples R China

年份：2026

卷号：9

期号：31

起止页码：14993

外文期刊名：ACS APPLIED NANO MATERIALS

收录：;EI(收录号：20263321301786);Scopus(收录号：2-s2.0-105047281555);WOS:【SCI-EXPANDED(收录号:WOS:001830578800001)】；

基金：We gratefully acknowledge funding from the National Natural Science Foundation of China (NSFC 92356301, 22378126, 22338006, and 22508119), the Basic Science Center of National Natural Science Foundation (T2488302), the Special Projects on Regional Collaborative Innovation-SCO Science and Technology Partnership Program, the International Science and Technology Cooperation Program (2025E01025), the Science and Technology Commission of Shanghai Municipality (25ZR1402115 and 25SF1904700), the Guangxi Science and Technology Innovation Platform Program ('' Leitai '' Action Plan, Guangxi Laboratory Capacity Building) (LT2504240015). M.Y. acknowledges the Shanghai "Super Postdoctoral Researchers '' program.

语种：英文

外文关键词：exosome nanoclusters; electrostatic assembly; charge convertible; cellular uptake; restenosis

摘要：Exosomes hold great promise for vascular disease therapy but suffer from rapid clearance, structural instability, and poor cellular uptake due to their small size and negative surface charge. Conventional delivery systems often involve harsh preparation conditions that compromise exosome integrity. Herein, we report a facile and mild strategy to construct exosome nanoclusters (exo NCs) via electrostatic assembly using the cationic polymer chitosan and polyethylene glycol-distearoylphosphatidylethanolamine (PEG-DSPE) in a confined impinging jet with dilution (CIJ-D) mixer. By optimizing key processing parameters, we obtained exo NCs with reversed surface charge and demonstrated improved stability in the bloodstream under the tested conditions. The resulting exo NCs showed significantly enhanced cellular uptake efficiency compared to exosomes. Moreover, this strategy exhibited effective inhibition of excessive proliferation and migration of vascular smooth muscle cells, promoted endothelial cell migration and tube formation, and showed improved therapeutic efficacy in a mouse model of carotid artery injury. Our findings suggest that this approach holds promise as a safe and scalable platform for exosome-based vascular therapy; however, further studies are warranted to fully establish its translational potential.