文献类型：期刊文献

英文题名：A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis

作者：Zhang, Zhen[1,2];Wang, Baobei[2,4];Hu, Qiang[3];Sommerfeld, Milton[2];Li, Yuanguang[1];Han, Danxiang[3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Arizona State Univ, Dept Human Syst & Environm, Mesa, AZ USA;[3]Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China;[4]Xiamen Univ, Dept Chem & Biochem Engn, Xiamen, Fujian, Peoples R China

年份：2016

卷号：113

期号：10

起止页码：2088

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20163602766819);WOS:【SCI-EXPANDED(收录号:WOS:000386753600002)】；

基金：This work is partially supproted by State Development & Investment Corporation (SDIC) of China, and National Science Foundation of Hubei Province (2015CFA022).

语种：英文

外文关键词：astaxanthin; Haematococcus pluvialis; heterotrophy; acclimation

摘要：The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark-grown cells. Althoughmoderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5mg L-1 day(-1)) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark-grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high-light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5mg L-1 day(-1) by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark-grown cells under photo-induction conditions. (C) 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.