文献类型：期刊文献

英文题名：Gene cloning and expression of a novel hypoglycaemic peptide from Momordica charantia

作者：Wang, Bi-lian[1];Zhang, Wen-ji[1];Zhao, Jian[1];Wang, Fu-jun[2];Fan, Li-qiang[1];Wu, Yi-xin[3];Hu, Zhi-bi[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Univ Chinese Tradit Med, Shanghai 201203, Peoples R China;[3]Hamamatsu Univ Sch Med, Dept Biochem, Hamamatsu, Shizuoka 4313125, Japan

年份：2011

卷号：91

期号：13

起止页码：2443

外文期刊名：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000295429600023)】；

基金：This work was financially supported by the National Key Project of Scientific and Technical Supporting Programs funded by the Ministry of Science and Technology of China (2006BAI06A16).

语种：英文

外文关键词：Momordica charantia; gene cloning; polypeptide-P; hypoglycaemic effect

摘要：BACKGROUND: Momordica charantia (MC) is used in many Asian countries as a traditional functional food and medicine. Polypeptide-P, a 166 amino acid (AA) polypeptide isolated from MC seeds, has been reported to show hypoglycaemic effects in patients with type I or type II diabetes. The AA sequence of this peptide has been determined, but its gene sequence has yet to be published. RESULTS: In this study a gene-cloning strategy was employed to obtain the polypeptide-P gene sequence using degenerate reverse transcription polymer chain reaction and genome-walking methods. A complete 498 bp sequence encoding the polypeptide-P protein was cloned from MC seeds. Subsequent assays of the bioactivity of the expressed recombinant protein revealed that it had significant hypoglycaemic activity in alloxan-induced diabetic mice. This result suggests that recombinant polypeptide-P has hypoglycaemic effects. CONCLUSION: This is the first report of cloning and expression of the MC polypeptide-P gene. The cloned gene could be helpful for exploring the mechanisms of polypeptide-P gene expression and regulation in MC. Furthermore, this gene could be used as a potential tool both for screening MC varieties with high hypoglycaemically active substance content and for breeding new varieties of MC with high economic value, which could in turn be beneficial to farmers. (C) 2011 Society of Chemical Industry