文献类型：期刊文献

中文题名：1,3-丙二醇发酵过程中盐浓度的胁迫作用

英文题名：1,3-Propanediol Production Under Salt Stress

作者：徐佳杰[1];刘朋波[1];陶春平[1];程骋[1];付水林[1];宫衡[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2008

卷号：24

期号：6

起止页码：1098

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：MEDLINE(收录号：18807999);CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：盐浓度;中试实验;发酵条件;氧化还原电位

外文关键词：salt concentration, middle industrial scale, fermentation conditions, oxidation reduction potential

摘要：通过对克雷伯氏菌(Klebsiella pneumoniae)甘油发酵生产1,3-丙二醇(1,3-PD)过程的研究发现,盐浓度对1,3-PD发酵有胁迫作用。盐浓度较低时,菌体生长和产物生成均维持较高速率;盐浓度较高时会导致菌体生长减慢,1,3-PD最终浓度,甘油到1,3-丙二醇的转化率降低,同时1,3-丙二醇氧化还原酶受到抑制。在5m3罐中控制合适的盐浓度可以提高1,3-PD的发酵水平,使1,3-PD的最终浓度达到64g/L,转化率61%,生产强度2.1g/(L·h)。
Through studying the process of glycerol fermentation to 1, 3-propanediol（1, 3-PD） by Klebsiella pneumoniae, it was found that the cell growth and product （or by-product） production were under salt stress. Cell growth and product formation kept high rate at low salt concentration. High salt concentration led to low growth of cells, final concentration of 1, 3-PD and conversion from glycerol to 1, 3-PD, and, 1, 3-propanediol oxidoreductase activity decreased. When the salt concentration in 5 m^3 bioreactor was controlled under appropriate manner, the concentration of 1, 3-PD production was markedly enhanced. The final 1, 3-PD concentration ,the conversion of glycerol to 1, 3-PD and productivity were 64 g/L, 61% and 2.1 g/（L · h）.