文献类型：期刊文献

英文题名：Roundup-Induced AMPK/mTOR-Mediated Autophagy in Human A549 Cells

作者：Hao, Youwu[1];Xu, Wenping[1];Gao, Jufang[2];Zhang, Yang[1];Yang, Yun[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China

年份：2019

卷号：67

期号：41

起止页码：11364

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20194207551737);WOS:【SCI-EXPANDED(收录号:WOS:000491220400010)】；

基金：This work was financially supported by National Key Technology Research Development Program of China (no. 2017YFD0200505). In addition, Y.H. especially wishes to thank JJ Lin, whose songs give him powerful spiritual support.

语种：英文

外文关键词：Roundup; adjuvant POEA; glyphosate isopropylamine salt; A549 cells; autophagy

摘要：The extensive use of pesticide caused an amount of pressure on the environment and increased the potential human health risk. Glyphosate-based herbicide (GBH) is one of the most widely used pesticides based on a 5-enolpyruvylshikimate-3-phosphate synthase target, which does not exist in vertebrates. Here, we study autophagic effects of the most famous commercial GBH Roundup (RDP) on human A549 cells in vitro. Intracellular biochemical assay indicated opening of mitochondrial permeability transition pore, LC3-II conversion, up-regulation of beclin-1, down-regulation of p62, and the changes in the phosphorylation of AMPK and mTOR induced by RDP in A549 cells. Further experimental results indicated that all the effects induced by RDP were related to its adjuvant polyethoxylated tallow amine, not its herbicidal active ingredient glyphosate isopropylamine salt. All these results showed that RDP has the ability to induce AMPK/mTOR-mediated cell autophagy in human A549 cells. This study would provide a theoretical basis for understanding RDP's autophagic effects on human A549 cells and attract attention on the potential human health risks induced by the adjuvant.