文献类型：期刊文献

英文题名：Efficient production of (R)-(-)-mandelic acid by isopropanol-permeabilized recombinant E. coli cells expressing Alcaligenes sp nitrilase

作者：Zhang, Zhi-Jun[1,2];Yu, Hui-Lei[1,2];Imanaka, Tadayuki[1,2,3];Xu, Jian-He[1,2]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Synthet Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China;[3]Ritsumeikan Univ, Coll Life Sci, Dept Biotechnol, Shiga 5258577, Japan

年份：2015

卷号：95

起止页码：71

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20150100401578);WOS:【SCI-EXPANDED(收录号:WOS:000349428000008)】；

基金：This work was financially supported by the National Science Foundation of China (Nos. 21276082 & 21406067), Ministry of Science and Technology, PR China (Nos. 2011CB710800, 2011AA02A210 & 2012AA022201), Shanghai Commission of Science and Technology (No. 11431921600), the Fundamental Research Funds for the Central Universities (No. 222201314016) and the China Postdoctoral Science Foundation (No. 2014M560309).

语种：英文

外文关键词：Biocatalysis; Alcaligenes sp nitrilase; Mass transfer; Cell permeabilization; Immobilised cells; Bioprocess design

摘要：Resting cells or immobilized cells are preferentially used as biocatalyst for biocatalytic processes due to the easy preparation and operation, however, the catalytic efficiency of biocatalyst is usually limited by the transport barrier of cell membrane. In this work, the transport barrier of recombinant Escherichia coli cells expressing the nitrilase from Alcaligenes sp. ECU0401 was relieved by isopropanol permeabilization. Under the optimal permeabilization conditions, the nitrilase activity of the permeabilized cells was 4.6-fold that of the cells without permeabilization. The permeabilized cells were evaluated for the enantioselective hydrolysis of mandelonitrile, which showed an initial reaction rate of about 2-fold that of the cells without permeabilization. The permeabilization process did not impair the stability of the cells and after simple immobilization by glutaraldehyde cross-linking, the imrliobilized permeabilized cells were successfully recycled for 15 runs without significant activity loss, suggesting the potential application of the immobilized permeabilized cells for the production of (R)-(-)-mandelic acid. The technology reported in this work might also be extended to other bioprocesses facing cell membrane transport barriers. (C) 2014 Elsevier B.V. All rights reserved.