文献类型：期刊文献

英文题名：Click novel glycosyl amino acid hydrophilic interaction chromatography stationary phase and its application in enrichment of glycopeptides

作者：Huang, Hongxue[1];Guo, Hongyue[1];Xue, Meiyun[1];Liu, Yajing[1];Yang, Jinjin[1];Liang, Xinmiao[1];Chu, Changhu[1]

机构：[1]E China Univ Sci & Technol, Shanghai 200237, Peoples R China

年份：2011

卷号：85

期号：3

起止页码：1642

外文期刊名：TALANTA

收录：;EI(收录号：20113214215042);WOS:【SCI-EXPANDED(收录号:WOS:000294092800061)】；

基金：We acknowledge the financial support from the Shanghai Natural Science Foundation (No. 11ZR1408500) and the National Natural Science Foundation of China (No. 21072055).

语种：英文

外文关键词：Glycosyl amino acid; Hydrophilic interaction chromatography; Glycopeptide enrichment

摘要：A novel glycosyl amino acid hydrophilic interaction chromatography (HILIC) stationary phase was prepared via click chemistry. The key intermediate N-3-glycosyl D-phenylglycine was prepared by a three steps procedure, including selective condensation of amino glucose with N-succinimidyl ester of Boc-D-phenylglycine, deprotection and transformation of amino group to azido group. The structure of all the intermediates and functionalized silica beads were confirmed by H-1 NMR, IR, elemental analysis and C-13 CP-MAS. The chromatography test showed that this new type of separation material possessed good HILIC properties and glycopeptide enrichment characteristics. Nucleosides and bases could be separated in a simple eluent composition (only acetonitrile in combined with water), and with the same condition, these model compounds could not be separated on the commercial HILIC column (Atlantis). Click glycosyl amino acid thus prepared also showed longer retention and better separation ability in the separation of polar organic acids. (C) 2011 Elsevier B.V. All rights reserved.