文献类型：期刊文献

中文题名：Simultaneous Determination of Saponins in Radix Glycyrrhizae, Notoginseng and Ginseng by High Performance Liquid Chromatography

英文题名：Simultaneous determination of saponins in Radix Glycyrrhizae, Notoginseng and Ginseng by high performance liquid chromatography

作者：Zhang, Liguo[1]; Fang, Congying[1]; Ouyang, Xiaowen[1]; Ni, Lijun[1]

机构：[1] School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2013

卷号：19

期号：6

起止页码：430

中文期刊名：Transactions of Tianjin University

外文期刊名：Transactions of Tianjin University

收录：EI(收录号：20140217173862);Scopus

基金：Supported by Plan Issue of Shanghai Science &Technology Committee(No.13401901100)

语种：英文

中文关键词：high performance liquid chromatography diode array detector Radix Glycyrrhizae Radix Notoginseng Radix Ginseng saponin

外文关键词：Column chromatography - Metabolites - Liquids

摘要：To establish a method for determining five saponins(notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ammonium glycyrrhizinate) in Glycyrrhizae, Notoginseng and Ginseng, the high performance liquid chromatography with diode array detector(HPLC-DAD) method was applied to an Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid in a gradient elution manner. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelengths were 203 nm and 237 nm, respectively. The linear ranges were 0.700,0—7.000,0 μg for R1(r=1.000,0), 0.751,1— 7.511,4 μg for Rg1(r=1.000,0), 0.677,2—6.771,6 μg for Re(r=1.000,0), 0.733,9—7.339,1 μg for Rb1(r= 1.000,0), and 0.540,0—5.399,8 μg for ammonium glycyrrhizinate(r=0.999,9), respectively. In addition, their average recoveries were 100.28%, 105.83%, 104.09%, 99.36% and 98.54%, respectively. The relative standard deviations(RSDs) of precision, reproducibility and recovery were all less than 1.5%. The results indicate that the method is simple, accurate and reproducible so that it can be used for the simultaneous determination of the five saponins in Chinese patent medicines containing the three kinds of herbs.
To establish a method for determining five saponins (notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ammonium glycyrrhizinate) in Glycyrrhizae, Notoginseng and Ginseng, the high performance liquid chromatography with diode array detector (HPLC-DAD) method was applied to an Inertsil ODS-SP column (4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid in a gradient elution manner. The flow rate was 1.0 mL/min. The column temperature was 30 C and the detection wavelengths were 203 nm and 237 nm, respectively. The linear ranges were 0.700 0-7.000 0 μg for R1 (r=1.000 0), 0.751 1-7.511 4 μg for Rg1 (r=1.000 0), 0.677 2-6.771 6 μg for Re (r=1.000 0), 0.733 9-7.339 1 μg for Rb1(r= 1.000 0), and 0.540 0-5.399 8 μg for ammonium glycyrrhizinate (r=0.999 9), respectively. In addition, their average recoveries were 100.28%, 105.83%, 104.09%, 99.36% and 98.54%, respectively. The relative standard deviations (RSDs) of precision, reproducibility and recovery were all less than 1.5%. The results indicate that the method is simple, accurate and reproducible so that it can be used for the simultaneous determination of the five saponins in Chinese patent medicines containing the three kinds of herbs. ? 2013 Tianjin University and Springer-Verlag Berlin Heidelberg.