文献类型：期刊文献

英文题名：Spectrometric study on the interaction of sodium cholate aggregates with quercetin

作者：Zhou, Haibo[1];Wang, Xiaoyong[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：481

起止页码：31

外文期刊名：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS

收录：;EI(收录号：20151900818569);WOS:【SCI-EXPANDED(收录号:WOS:000360407700005)】；

基金：This work is sponsored by the National Natural Science Foundation of China (Grant 21173081) and the Fundamental Research Funds for the Central Universities (Grant WK1213003).

语种：英文

外文关键词：Bile salt; Quercetin; Micelles; Fluorescence; Hydrophobic interaction

摘要：In this work, the interaction between sodium cholate (NaC) aggregates and quercetin has been studied in pH 7.4 sodium phosphate buffer. Surface tension measurement reveals that the presence of quercetin leads NaC to have increased critical aggregation concentrations, owing to the electrostatic repulsion between negative charged NaC and anionic quercetin species. As NaC monomers gradually aggregate into dimers, primary and secondary micelles, quercetin mixed with NaC often gives higher intensities of absorption and fluorescence than free quercetin, which is explained in terms of the hydrophobic binding of quercetin with NaC. The measurement of quercetin anisotropy suggests that quercetin experiences a more hydrophobic microenvironment in NaC secondary micelles than those in NaC dimers and primary micelles. The binding constant of quercetin with NaC secondary micelles is found to be higher than the value of quercetin with NaC primary micelles, which is ten times that of quercetin with NaC dimers. Moreover, NaC secondary micelles are superior to NaC dimers and primary micelles for enhancing the radical scavenging ability of quercetin. (C) 2015 Elsevier B.V. All rights reserved.