文献类型：期刊文献

英文题名：The Influence of Temperature on Stationary Phase Retention in Countercurrent Chromatography: Integrating Experiment With an Extended Du's Plot Model

作者：Zhao, Peishan[1];Yuan, Mengjiao[1];Liu, Yan[2];Ignatova, Svetlana[3];Zhang, Hongyang[4,5];Wang, Yuerong[4,5];Hu, Ping[4,5];Zhang, Min[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai, Peoples R China;[3]Brunel Univ London, Dept Chem Engn, CEDPS, Uxbridge, England;[4]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, Dept Chem, Shanghai, Peoples R China

年份：2026

卷号：49

期号：3

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20261120283950);WOS:【SCI-EXPANDED(收录号:WOS:001715164400001)】；

基金：The authors acknowledge the financial support from the National Natural Science Foundation of China (Nos. 81573397 and 81973285).

语种：英文

外文关键词：countercurrent chromatography; physicochemical properties; solvent system; stationary phase retention; temperature effect

摘要：The selection of a biphasic solvent system is the first and most important step for a successful countercurrent chromatography (CCC) separation. The partitioning coefficient of targets and their related impurities on one side, and the stationary phase retention on the other, are among the parameters used for solvent system selection. At constant temperature, the stationary phase retention of a selected solvent system in any CCC instrument is dominated by the rule of Du's plot, which describes the correlation between stationary phase retention and flow rate. In a biphasic liquid system, physico-chemical properties such as density, viscosity, and interfacial tension between two phases are temperature dependent. In this article, the effect of temperature on the retention of several typical solvent systems was investigated using a high-speed CCC (HSCCC) instrument. The results showed that temperature changes could induce density, viscosity, and interfacial tension variation of the biphasic system with consequent changes in the system's hydrodynamic behavior. As the temperature increased, the density and viscosity of the selected solvent system decreased. This led to a variation in the interfacial tension between two phases depending on the solvent system composition. The stationary phase retention increased when the temperature increased for the tested solvent systems. Based on the obtained results, an improved Du's plot was suggested, accounting for the contribution of operating temperature. The prospect of this article provides hands-on strategies for the development of high-performance separation method for HSCCC.