文献类型：期刊文献

英文题名：Genetic engineering of the branched-chain fatty acid biosynthesis pathway to enhance surfactin production from Bacillus subtilis

作者：Jing, Ya-Fei[1,2];Wei, Hao-Xun[1,2];Liu, Fang-Fang[1,2];Liu, Yi-Fan[1,2];Zhou, Lei[1,2];Liu, Jin-Feng[1,2,3];Yang, Shi-Zhong[1,2,3,4];Zhang, Hui-Zhan[1,2];Mu, Bo-Zhong[1,2,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[3]Minist Educ, Engn Res Ctr Microbial Enhanced Oil Recovery, Shanghai, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai, Peoples R China

年份：2023

卷号：70

期号：1

起止页码：238

外文期刊名：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

收录：;EI(收录号：20221812045199);WOS:【SCI-EXPANDED(收录号:WOS:000788465600001)】；

基金：This work was supported by funding from the National Key Research and Development Program of China (No. 2017YFB0308900), the National Natural Science Foundation of China (No. 51574125), and the Fundamental Research Funds for the Central Universities of China (No. JKJ01211714).

语种：英文

外文关键词：Bacillus subtilis; fatty acid; gene expression; metabolic engineering; precursor biosynthesis; surfactin

摘要：Surfactin, which is composed of a beta-hydroxy fatty acid chain and a peptide ring, has drawn considerable attention due to its potential applications in the biomedicine, bioremediation, and petroleum industries. However, the low yield of surfactin from wild strains still restricts its industrial applications. In this study, eight genes relevant to the fatty acid biosynthesis pathway were targeted to enhance surfactin production, and high surfactin-yielding strains with potential industrial applications were obtained. When ldeHA and acc were co-overexpressed, the surfactin yield of recombinant strains TDS8 and TPS8 increased to 1.55- and 1.19-fold of their parental strains, respectively, again proving that the conversion of acetyl-coenzyme A (CoA) to malonyl-CoA is the rate-limiting step in fatty acid biosynthesis. Furthermore, changes in surfactin isoforms of recombinant strain TPS8 suggest that the fatty acid precursor synthesis pathway can be modified to improve the proportion of different isoforms. In addition, the deletion of lpdV, which is responsible for the conversion of alpha-ketoacyl-CoA precursors, resulted in a sharp decrease in surfactin production, further demonstrating the importance of branched-chain fatty acid biosynthesis in surfactin production. This work will facilitate the design and construction of more efficiently engineered strains for surfactin production and further extend industrial applications.