文献类型：期刊文献

英文题名：Lignin Removal in Subcellular Location of Poplar Cell Wall During Pretreatment Significantly Impacts Cellulose Digestibility

作者：Zhang, Jian[1,2,3];Kang, Lin[2,3,4,5];Shen, Wei[2,3];Collings, Cynthia[2,3];Gong, Heng[1];vander Meulen, Kirk[6,7];Fox, Brian G.[6,7];Gilcher, Elise[6,8];Dumesic, James A.[6,8];Ding, Shi-You[2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA;[3]Michigan State Univ, DOE Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA;[4]Chinese Acad Sci, Xiamen Inst Rare Earth Mat, Haixi Inst, Xiamen Key Lab Rare Earth Photoelect Funct Mat, Xiamen 361021, Fujian, Peoples R China;[5]Chinese Acad Sci, Fujian Inst Res Struct Matter, Fuzhou 350002, Fujian, Peoples R China;[6]DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA;[7]Univ Wisconsin Madison, Dept Biochem, Madison, WI 53706 USA;[8]Univ Wisconsin Madison, Dept Chem & Biol Engn, Madison, WI 53706 USA

年份：2025

卷号：13

期号：21

起止页码：7781

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20252118476687);WOS:【SCI-EXPANDED(收录号:WOS:001491819800001)】；

基金：This work was supported by U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, under Award Number DE-SC0019072 and by the Great Lakes Bioenergy Research Center (DE-SC0018409). J.Z. would like to thank Natural Science Foundation of China (22178105) for supporting this research. L.K. would like to thank Natural Science Foundation of Xiamen, China (3502Z202472049). S.-Y.D. also acknowledges support of the National Science Foundation under Grant No. 2321398. The authors acknowledge Novozymes (Franklinton, NC, USA) for kindly providing the enzymes.

语种：英文

外文关键词：GVL-HCl pretreatment; lignin removal; subcellular location; CBM-GFP binding; stimulatedRaman scattering microscopy (SRS); enzyme digestibility

摘要：The gamma-valerolactone (GVL) pretreatment is one of the leading solvent-based methods for producing high-quality lignin under mild conditions. However, the glucan conversion yield from GVL pretreated biomass remains unsatisfactory. To explore the discrepancies between the relatively low glucan conversion and high lignin extraction, we conducted GVL-HCl and NaOH pretreatments on poplar and investigated their effects on lignin content and location, as well as on enzymatic hydrolysis of poplar cell walls at the subcellular level. Under designated pretreatment conditions of GVL-HCl (90% GVL, 0.1 M HCl, 100 degrees C, 1 h) and NaOH (1 M, 121 degrees C, 2 h), the glucan conversion yields were 69.4% and 95.8%, with lignin removal rates of 67.8% and 47.7%, respectively. Four types of GFP-labeled carbohydrate binding modules were used to identify different forms of cellulose in the pretreated cell walls. The overall binding intensities to pretreated poplar were stronger for NaOH compared to GVL-HCl pretreatment. Stimulated Raman scattering microscopy imaging revealed that GVL-HCl preferentially extracted lignin from the compound middle lamella and cell corner areas, while NaOH effectively dissolved lignin in the secondary cell walls. Real-time imaging of cellulase degradation of pretreated cell walls further indicated that digestion started from both the cell lumen and the compound middle lamella areas for GVL, whereas it occurred uniformly across the secondary cell walls for NaOH. Our findings suggest that the location of lignin removal during pretreatment is crucial for enzymatic cellulose degradation, in addition to the total amount of lignin extraction.